Analysis of joint protein expression profile in anterior disc displacement of TMJ with or without OA.

IF 2.9 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-22 DOI:10.1111/odi.14871
Luxiang Zou, Kaiwen Yang, Yeke Yu, Chuyao Wang, Jieyun Zhao, Chuan Lu, Dongmei He
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引用次数: 0

Abstract

Objective: Anterior disc displacement (ADD) is a common clinical issue and may cause osteoarthritis (OA). However, the research of protein changes in synovial fluid as disease development marker and potential treatment clue is still insufficient.

Materials and methods: We conducted the high-resolution mass spectrometry (MS) of synovial fluid collected from 60 patients with normal disk position to ADD and ADD with osteoarthritis (OA). The proteins with significant changes among the 3 groups were analyzed by biological information and further validated by in primary rat condyle chondrocytes and OA animal model.

Results: FGL2, THBS4, TNC, FN1, OMD etc. were significantly increased in ADD without OA (p < 0.05), which reflected the active extracellular matrix and collagen metabolism. FGFR1, FBLN2, GRB2 etc. were significantly increased in ADD with OA group (p < 0.05), which revealed an association with apoptosis and ferroptosis. Proteins such as P4HB, CBLN4, FHL1, VIM continuously increase in the whole disease progress (p < 0.05). Both the in vitro and in vivo results are consistent with protein changes detected in MS profile.

Conclusion: This study firstly provides the expression changes of proteins from normal disc condyle relationship toward ADD with OA, which can be selected and studied further as disease progress marker and potential treatment targets.

颞下颌关节前盘移位伴或不伴 OA 的关节蛋白表达谱分析。
目的:椎间盘前移位(ADD)是一种常见的临床问题,可能导致骨关节炎(OA)。然而,将滑液中蛋白质的变化作为疾病发展标志和潜在治疗线索的研究仍然不足:我们对 60 名椎间盘位置正常的 ADD 患者和患有骨关节炎(OA)的 ADD 患者的滑液进行了高分辨率质谱分析。通过生物信息分析了3组之间有显著变化的蛋白质,并通过原代大鼠髁突软骨细胞和OA动物模型进行了进一步验证:结果:FGL2、THBS4、TNC、FN1、OMD 等蛋白在无 OA 的 ADD 中明显增加(p 结论:该研究首次提供了大鼠髁突软骨细胞和 OA 动物模型的蛋白表达变化:该研究首次提供了正常椎间盘髁突关系蛋白在伴有OA的ADD中的表达变化,可作为疾病进展标志物和潜在治疗靶点进行筛选和进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Oral diseases
Oral diseases 医学-牙科与口腔外科
CiteScore
7.60
自引率
5.30%
发文量
325
审稿时长
4-8 weeks
期刊介绍: Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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