Isolation and whole-genome sequencing analysis of Escherichia fergusonii harboring a heat-labile enterotoxin gene from retail chicken meat in Okinawa, Japan

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2024-01-20 DOI:10.1111/1348-0421.13115

Tetsuya Kakita, Kenichi Lee, Masatomo Morita, Miki Okuno, Hisako Kyan, Sho Okano, Noriyuki Maeshiro, Momoko Ishizu, Tsuyoshi Kudeken, Haruno Taira, Morimi Teruya, Yoshitoshi Ogura, Yukihiro Akeda, Makoto Ohnishi

引用次数: 0

Abstract

This study aimed to reveal the prevalence of heat-labile enterotoxin (LT) gene-positive Escherichia fergusonii in retail chicken meat and genetically characterize these strains. E. fergusonii harboring LT gene was isolated from 6 out of 60 (10%) retail chicken samples in Okinawa, Japan. Whole-genome sequencing analysis revealed that LT gene-positive E. fergusonii from chicken meat and feces contain an IncFII plasmid harboring elt1AB, and suggested to spread clonally to retail chicken through fecal contamination. Additionally, it was found that these strains harbor multidrug-resistant genes on their plasmids. Their pathogenicity and continuous monitoring are required for confirmation.

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从日本冲绳零售鸡肉中分离出携带热嗜性肠毒素基因的弗氏大肠杆菌并对其进行全基因组测序分析。

本研究旨在揭示零售鸡肉中热嗜性肠毒素（LT）基因阳性的弗氏大肠杆菌的普遍性，并从遗传学角度分析这些菌株的特征。在日本冲绳的 60 份零售鸡肉样本中，有 6 份（10%）分离出了携带 LT 基因的弗氏大肠杆菌。全基因组测序分析表明，从鸡肉和粪便中检出的LT基因阳性的铁古逊酵母菌含有一个携带elt1AB的IncFII质粒，这表明它们通过粪便污染克隆传播到零售鸡肉中。此外，研究还发现这些菌株的质粒上含有耐多种药物的基因。它们的致病性需要持续监测才能确认。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.