A monoclonal antibody collection for C. difficile typing ?

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2024-01-19 DOI:10.1186/s13099-023-00592-7

Lise Hunault, Patrick England, Frédéric Barbut, Bruno Iannascoli, Ophélie Godon, François Déjardin, Christophe Thomas, Bruno Dupuy, Chunguang Guo, Lynn Macdonald, Guy Gorochov, Delphine Sterlin, Pierre Bruhns

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Abstract

Clostridioides difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis in adults. Various C. difficile strains circulate currently, associated with different outcomes and antibiotic resistance profiles. However, most studies still focus on the reference strain 630 that does not circulate anymore, partly due to the lack of immunological tools to study current clinically important C. difficile PCR ribotypes. The goal of this study was to generate monoclonal antibodies recognizing various epidemic ribotypes of C. difficile. To do so, we immunized mice expressing human variable antibody genes with the Low Molecular Weight (LMW) subunit of the surface layer protein SlpA from various C. difficile strains. Monoclonal antibodies purified from hybridomas bound LMW with high-affinity and whole bacteria from current C. difficile ribotypes with different cross-specificities. This first collection of anti-C. difficile mAbs represent valuable tools for basic and clinical research.

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用于艰难梭菌分型的单克隆抗体集 ?

艰难梭菌是导致成人抗生素相关性腹泻和假膜性结肠炎的主要原因。目前有多种不同的艰难梭菌菌株流行，其结果和抗生素耐药性各不相同。然而，大多数研究仍侧重于不再流通的参考菌株 630，部分原因是缺乏免疫学工具来研究当前临床上重要的艰难梭菌 PCR 核型。本研究的目标是产生能识别艰难梭菌各种流行核型的单克隆抗体。为此，我们用来自不同艰难梭菌菌株的表面层蛋白 SlpA 的低分子量（LMW）亚基免疫表达人类可变抗体基因的小鼠。从杂交瘤中纯化的单克隆抗体以不同的交叉特异性高亲和力结合了艰难梭菌核型的低分子量和全菌。这第一批抗艰难梭菌 mAbs 是基础和临床研究的宝贵工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).