rDNA transcription, replication and stability in Saccharomyces cerevisiae

IF 6.2 2区生物学 Q1 CELL BIOLOGY

Seminars in cell & developmental biology Pub Date : 2024-01-19 DOI:10.1016/j.semcdb.2024.01.004

Anna D’Alfonso , Gioacchino Micheli , Giorgio Camilloni

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引用次数: 0

Abstract

The ribosomal DNA locus (rDNA) is central for the functioning of cells because it encodes ribosomal RNAs, key components of ribosomes, and also because of its links to fundamental metabolic processes, with significant impact on genome integrity and aging. The repetitive nature of the rDNA gene units forces the locus to maintain sequence homogeneity through recombination processes that are closely related to genomic stability. The co-presence of basic DNA transactions, such as replication, transcription by major RNA polymerases, and recombination, in a defined and restricted area of the genome is of particular relevance as it affects the stability of the rDNA locus by both direct and indirect mechanisms. This condition is well exemplified by the rDNA of Saccharomyces cerevisiae. In this review we summarize essential knowledge on how the complexity and overlap of different processes contribute to the control of rDNA and genomic stability in this model organism.

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酿酒酵母中 rDNA 的转录、复制和稳定性

核糖体 DNA 基因座（rDNA）是细胞功能的核心，因为它编码核糖体的关键成分核糖体 RNA，还因为它与基本代谢过程有关，对基因组的完整性和衰老有重大影响。rDNA 基因单位的重复性迫使基因座通过与基因组稳定性密切相关的重组过程来保持序列的同质性。在基因组的一个确定和受限的区域内同时存在基本的 DNA 事务，如复制、主要 RNA 聚合酶的转录和重组，这一点特别重要，因为它会通过直接和间接的机制影响 rDNA 基因座的稳定性。酿酒酵母（Saccharomyces cerevisiae）的 rDNA 就很好地说明了这种情况。在这篇综述中，我们总结了关于不同过程的复杂性和重叠性如何有助于控制这种模式生物的 rDNA 和基因组稳定性的基本知识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Seminars in cell & developmental biology 生物-发育生物学

CiteScore

15.10

自引率

1.40%

发文量

310

审稿时长

9.1 weeks

期刊介绍： Seminars in Cell and Developmental Biology is a review journal dedicated to keeping scientists informed of developments in the field of molecular cell and developmental biology, on a topic by topic basis. Each issue is thematic in approach, devoted to an important topic of interest to cell and developmental biologists, focusing on the latest advances and their specific implications. The aim of each issue is to provide a coordinated, readable, and lively review of a selected area, published rapidly to ensure currency.