Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells.

Joseph M Heili, Kaitlin Stokes, Nathaniel J Gaut, Christopher Deich, Judee Sharon, Tanner Hoog, Jose Gomez-Garcia, Brock Cash, Matthew R Pawlak, Aaron E Engelhart, Katarzyna P Adamala
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Abstract

Synthetic minimal cells are a class of bioreactors that have some, but not all, functions of live cells. Here, we report a critical step toward the development of a bottom-up minimal cell: cellular export of functional protein and RNA products. We used cell-penetrating peptide tags to translocate payloads across a synthetic cell vesicle membrane. We demonstrated efficient transport of active enzymes and transport of nucleic acid payloads by RNA-binding proteins. We investigated influence of a concentration gradient alongside other factors on the efficiency of the translocation, and we show a method to increase product accumulation in one location. We demonstrate the use of this technology to engineer molecular communication between different populations of synthetic cells, to exchange protein and nucleic acid signals. The synthetic minimal cell production and export of proteins or nucleic acids allows experimental designs that approach the complexity and relevancy of natural biological systems. A record of this paper's transparent peer review process is included in the supplemental information.

合成最小细胞之间蛋白质和核酸信号的可控交换。
合成最小细胞是一类生物反应器,具有活细胞的部分功能,但不是全部功能。在这里,我们报告了向开发自下而上的最小细胞迈出的关键一步:功能性蛋白质和 RNA 产物的细胞输出。我们利用细胞穿透肽标签将有效载荷转运过合成细胞囊膜。我们证明了活性酶的高效转运以及 RNA 结合蛋白对核酸有效载荷的转运。我们研究了浓度梯度和其他因素对转运效率的影响,并展示了一种在一个位置增加产物积累的方法。我们展示了如何利用这项技术在不同的合成细胞群之间进行分子交流,交换蛋白质和核酸信号。通过合成最小细胞生产和输出蛋白质或核酸,可以进行接近自然生物系统复杂性和相关性的实验设计。补充信息中包含了本文透明的同行评审过程记录。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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