Phylogenetic analysis of Acanthamoeba isolated from soil samples and nasal cavities of patients with malignancy: a public health concern in the northwest of Iran.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2024-06-04 DOI:10.1093/trstmh/trad100

Ali Pezeshki, Shadi Tajic, Parvin Farahmandian, Ali Haniloo, Abbas Mahmmodzadeh, Maryam Niyyati, Hamed Behniafar

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引用次数: 0

Abstract

Background: The genus Acanthamoeba is reported from various environmental sources and can cause multiple complications, including chronic amoebic aeratitis and amoebic granulomatous encephalitis. This study investigated the presence and genotyping of Acanthamoeba in the soil of parks and patients with malignancies referred to health centers in Zanjan city, Iran.

Methods: In this cross-sectional study, 200 soil samples were collected from amusement parks in Zanjan city from September 2017 to May 2018. Samples were cultured on 1.5% non-nutrient agar, and the Acanthamoeba genus was identified using the morphological method. PCR was performed on all positive environmental samples, and six microscopically positive clinical samples belonged to our previous study. DNA sequencing of 18S rRNA was performed to analyze the genetic pattern of some PCR-positive isolates.

Results: Microscopic results showed that 96 (48%) soil samples were positive. PCR confirmed all positive cases of clinical samples and 84 soil samples. Out of the PCR-positive samples, 20 soil samples and five clinical samples were sequenced successfully. All soil isolates belonged to the T4 genotype, and three and two clinical samples belonged to T4 and T5 genotypes, respectively.

Conclusion: : The presence of Acanthamoeba in both the environment and clinical samples of Zanjan city suggests paying greater attention to the infections caused by it.

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从土壤样本和恶性肿瘤患者鼻腔中分离的棘阿米巴的系统发育分析：伊朗西北部的公共卫生问题。

背景：据报道，阿卡阿米巴属来自各种环境来源，可引起多种并发症，包括慢性阿米巴气管炎和阿米巴肉芽肿性脑炎。本研究调查了伊朗赞詹市公园土壤和转诊到医疗中心的恶性肿瘤患者体内阿卡阿米巴的存在和基因分型情况：在这项横断面研究中，从 2017 年 9 月至 2018 年 5 月，从赞詹市的游乐园收集了 200 份土壤样本。样本在 1.5% 非营养琼脂上进行培养，并使用形态学方法确定棘阿米巴属。对所有阳性环境样本进行了聚合酶链式反应（PCR），6 份显微镜下阳性的临床样本属于我们之前的研究。对部分 PCR 阳性分离物进行了 18S rRNA 的 DNA 测序，以分析其遗传模式：显微镜结果显示，96 份（48%）土壤样本呈阳性。PCR 证实了所有临床样本和 84 份土壤样本的阳性病例。在 PCR 阳性样本中，有 20 份土壤样本和 5 份临床样本成功测序。所有土壤分离物均属于 T4 基因型，3 份和 2 份临床样本分别属于 T4 和 T5 基因型：赞詹市的环境和临床样本中都存在棘阿米巴，这表明应更加重视棘阿米巴引起的感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.