Omics approaches to understand the MADS-box gene family in common bean (Phaseolus vulgaris L.) against drought stress.

IF 2.5 3区生物学 Q3 CELL BIOLOGY

Protoplasma Pub Date : 2024-07-01 Epub Date: 2024-01-19 DOI:10.1007/s00709-024-01928-z

Aybüke Okay, Tarık Kırlıoğlu, Yasin Şamil Durdu, Sanem Şafak Akdeniz, İlker Büyük, E Sümer Aras

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引用次数: 0

Abstract

MADS-box genes are known to play important roles in diverse aspects of growth/devolopment and stress response in several plant species. However, no study has yet examined about MADS-box genes in P. vulgaris. In this study, a total of 79 PvMADS genes were identified and classified as type I and type II according to the phylogenetic analysis. While both type I and type II PvMADS classes were found to contain the MADS domain, the K domain was found to be present only in type II PvMADS proteins, in agreement with the literature. All chromosomes of the common bean were discovered to contain PvMADS genes and 17 paralogous gene pairs were identified. Only two of them were tandemly duplicated gene pairs (PvMADS-19/PvMADS-23 and PvMADS-20/PvMADS-24), and the remaining 15 paralogous gene pairs were segmentally duplicated genes. These duplications were found to play an important role in the expansion of type II PvMADS genes. Moreover, the RNAseq and RT-qPCR analyses showed the importance of PvMADS genes in response to drought stress in P. vulgaris.

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通过 Omics 方法了解蚕豆（Phaseolus vulgaris L.）MADS-box 基因家族对干旱胁迫的抗性。

众所周知，MADS-box 基因在多种植物的生长/发育和胁迫反应中发挥着重要作用。然而，还没有研究考察过粗壮褐藻中的 MADS-box 基因。本研究共鉴定了 79 个 PvMADS 基因，并根据系统进化分析将其分为 I 型和 II 型。虽然发现 I 型和 II 型 PvMADS 类都含有 MADS 结构域，但发现 K 结构域只存在于 II 型 PvMADS 蛋白中，这与文献报道一致。发现芸豆的所有染色体都含有 PvMADS 基因，并鉴定出 17 对同源基因。其中只有两个是串联重复的基因对（PvMADS-19/PvMADS-23 和 PvMADS-20/PvMADS-24），其余 15 个同源基因对都是片段重复的基因。研究发现，这些重复基因在 II 型 PvMADS 基因的扩增中发挥了重要作用。此外，RNAseq 和 RT-qPCR 分析表明了 PvMADS 基因在应对干旱胁迫中的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protoplasma 生物-细胞生物学

CiteScore

6.60

自引率

6.90%

发文量

审稿时长

4-8 weeks

期刊介绍： Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields: cell biology of both single and multicellular organisms molecular cytology the cell cycle membrane biology including biogenesis, dynamics, energetics and electrophysiology inter- and intracellular transport the cytoskeleton organelles experimental and quantitative ultrastructure cyto- and histochemistry Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".