Microscopic and molecular investigation of vector borne haemoprotozoan diseases in dromedary camel of North Gujarat.

IF 0.8 4区医学 Q4 INFECTIOUS DISEASES

Journal of Vector Borne Diseases Pub Date : 2024-01-16 DOI:10.4103/0972-9062.393968

Dhyanjyoti Sarma, Bhupamani Das, Niral Patel, Arun Patel, Abhinav Suthar, Ankit Prajapati, R M Patel

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引用次数: 0

Abstract

Background objectives: Vector borne haemoprotozoan diseases comprise diverse group of single celled organism transmitted by haematophagus invertebrates. The current study was aimed at identification of major haemoprotozoan (Babesia, Theileria and Trypanosoma) in dromedary camel of North Gujarat region using microscopy and Polymerase Chain Reaction (PCR).

Methods: A total of 234 blood samples were screened by the microscopic and molecular detection assays. Molecular prevalence studies of Theileria, Trypanosoma spp and Babesia was undertaken using 18s ribosomal DNA, RoTat 1.2 and SS rRNA gene respectively. The data relating to microscopic and molecular prevalence along with associated risk factors were analysed by statistical methods.

Results: The overall prevalence of hamoprotozoan disease based on microscopic and molecular investigation was 23.50%. The sensitivity and specificity (95% Confidence Interval) of PCR assay was 100% in comparison to microscopy (45.45% and 100%). The kappa coefficient between PCR and microscopy indicated good level of agreement with a value of 0.704 and SE of 0.159.

Interpretation conclusion: Despite holding much significance to the animal sector, little work has been undertaken in regional part of India regarding camel parasites. The present paper offers the first preliminary research data investigating haemoprotozoan disease using parasitological and molecular methods in camels in the region.

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北古吉拉特单峰骆驼病媒血吸虫疾病的显微镜和分子研究。

背景目标：病媒传播的血吸虫疾病包括由食血无脊椎动物传播的各种单细胞生物。本研究旨在利用显微镜和聚合酶链式反应（PCR）鉴定北古吉拉特地区单峰骆驼中的主要血吸虫（巴贝西亚原虫、泰勒氏原虫和锥虫）：方法：通过显微镜和分子检测方法共筛查了 234 份血液样本。分别使用 18s 核糖体 DNA、RoTat 1.2 和 SS rRNA 基因对 Theileria、Trypanosoma spp 和 Babesia 进行了分子流行研究。通过统计方法分析了显微和分子流行率数据以及相关风险因素：根据显微镜和分子检测结果，仓鼠疾病的总发病率为 23.50%。与显微镜检查（45.45% 和 100%）相比，PCR 检测的敏感性和特异性（95% 置信区间）均为 100%。PCR 与显微镜检查之间的卡帕系数（kappa coefficient）为 0.704，SE 为 0.159，表明两者的一致性很好：尽管骆驼寄生虫对畜牧业具有重要意义，但印度地区在骆驼寄生虫方面开展的工作却很少。本文首次提供了使用寄生虫学和分子方法调查该地区骆驼血吸虫病的初步研究数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vector Borne Diseases INFECTIOUS DISEASES-PARASITOLOGY

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： National Institute of Malaria Research on behalf of Indian Council of Medical Research (ICMR) publishes the Journal of Vector Borne Diseases. This Journal was earlier published as the Indian Journal of Malariology, a peer reviewed and open access biomedical journal in the field of vector borne diseases. The Journal publishes review articles, original research articles, short research communications, case reports of prime importance, letters to the editor in the field of vector borne diseases and their control.