Identification and function of the Pax gene Bmgsb in the silk gland of Bombyx mori

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Molecular Biology Pub Date : 2024-01-18 DOI:10.1111/imb.12886

Hongguo Yang, Yongping Xu, Yutong Yuan, Xuebing Liu, Jikailang Zhang, Jiaojiao Li, Ran Zhang, Jun Cao, Tingcai Cheng, Chun Liu

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Abstract

Paired box (Pax) genes are highly conserved throughout evolution, and the Pax protein is an important transcription factor of embryonic development. The Pax gene Bmgsb is expressed in the silk glands of silkworm, but its biological functions remain unclear. This study aimed to investigate the expression pattern of Bmgsb in the silk gland and explore its functions using RNA interference (RNAi). Here, we identified eight Pax genes in Bombyx mori. Phylogenetic analysis showed that the B. mori Pax genes were highly homologous to the Pax genes in other insects and highly evolutionarily conserved. The tissue expression profile showed that Bmgsb was expressed in the anterior silk gland and anterior part of the middle silk gland (AMSG). RNAi of Bmgsb resulted in defective development of the AMSG, and the larvae were mostly unable to cocoon in the wandering stage. RNA-seq analysis showed that the fibroin genes fib-l, fib-h and p25, cellular heat shock response-related genes and phenol oxidase genes were considerably upregulated upon Bmgsb knockdown. Furthermore, quantitative reverse transcription-PCR results showed that the fibroin genes and ubiquitin proteolytic enzyme-related genes were significantly upregulated in the AMSG after Bmgsb knockdown. This study provides a foundation for future research on the biological functions of B. mori Pax genes. In addition, it demonstrates the important roles of Bmgsb in the transcriptional regulation of fibroin genes and silk gland development.

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森蚕丝腺中 Pax 基因 Bmgsb 的鉴定和功能。

配对盒（Pax）基因在整个进化过程中高度保守，Pax 蛋白是胚胎发育的重要转录因子。Pax基因Bmgsb在家蚕丝腺中表达，但其生物学功能尚不清楚。本研究旨在利用 RNA 干扰（RNAi）技术研究 Bmgsb 在蚕丝腺中的表达模式并探索其功能。在此，我们在桑蚕中发现了 8 个 Pax 基因。系统进化分析表明，桑蚕的Pax基因与其他昆虫的Pax基因高度同源，在进化上高度保守。组织表达谱显示，Bmgsb在前丝腺和中丝腺（AMSG）前部表达。对Bmgsb的RNAi导致AMSG发育缺陷，幼虫在游走期大多不能结茧。RNA-seq分析表明，Bmgsb基因敲除后，纤维蛋白基因fib-l、fib-h和p25、细胞热休克反应相关基因和苯酚氧化酶基因均显著上调。此外，反转录-PCR定量分析结果表明，Bmgsb基因敲除后，AMSG中的纤维蛋白基因和泛素蛋白水解酶相关基因明显上调。这项研究为今后研究 B. mori Pax 基因的生物学功能奠定了基础。此外，它还证明了 Bmgsb 在纤维蛋白基因转录调控和丝腺发育中的重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).