A metabolomic snapshot through NMR revealed differences in phase transition during the induction of reproduction in Ulva ohnoi (Chlorophyta)†

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2024-01-19 DOI:10.1039/D3MO00197K

Payal A. Bodar, Rajendra Singh Thakur, Jasmine V. Rajai, Satej Bhushan and Vaibhav A. Mantri

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引用次数: 0

Abstract

The present study deals with the metabolomic status of Ulva cells undergoing phase transition (vegetative, determination and differentiation) when exposed to different abiotic conditions. The objective was to study whether metabolite changes occurring during the phase transition reveal any commonality among differential abiotic conditions. The phase transition was followed through microscopic observations and ¹H NMR characterization at 0 h, 24 h, and 48 h after the incubation of the thallus under abiotic conditions, such as different salinities (20–35 psu), temperatures (20–35 °C), photoperiods (18 : 6, 12 : 12, and 6 : 18 D/N), light intensities (220, 350, and 500 μmol photons m⁻² s⁻¹), nitrate (0.05–0.2 g L⁻¹) and phosphate (0.05–0.2 g L⁻¹) concentrations. Microscopic analysis revealed the role of all abiotic conditions except variable salinity and phosphate concentration in phase transition. NMR analysis revealed that glucose increased in the determination phase [7.58 to 9.62 normalized intensity (AU)] and differentiation phase (5.85 to 6.41 AU) from 20 °C to 25 °C temperature. Coniferyl aldehyde increased in vegetative (5.79 to 6.83 AU) and differentiation (6.66 to 7.40 AU) phases from 20 °C to 30 °C temperature. The highest average (22.97) was found in photoperiod (average range = 0–122.91) and the highest SD (24.73) in salinity (SD range = 1.86–57.04) in region 9 (creatinine and cysteine) of the differentiation phase. A total of 30 metabolites were identified under the categories of sugars, amino acids, and aromatic compounds. The present study will aid in understanding the mechanisms underlying cell differentiation during reproduction. The result may serve as an important reference point for future studies, besides helping in controlling seedling preparation for commercial farming as well as the management of rapid green tide formation.

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通过核磁共振进行的代谢组快照显示了大野莼（叶绿体）在生殖诱导过程中的相变差异。

本研究探讨了莼菜细胞在不同非生物条件下进行阶段转换（无性、确定和分化）时的代谢组学状况。目的是研究相变过程中发生的代谢物变化是否揭示了不同非生物条件下的共性。在不同的非生物条件下，如盐度（20-35 psu）、温度（20-35 °C）、光周期（18 : 6、12 : 12 和 6 : 18 D/N）、光照强度（220、350 和 500 μmol photons m-2 s-1）、硝酸盐（0.在这些条件下，水体中的硝酸盐（0.05-0.2 克/升-1）和磷酸盐（0.05-0.2 克/升-1）的浓度都会增加。显微分析表明，除了盐度和磷酸盐浓度的变化外，所有非生物条件都对相变起了作用。核磁共振分析表明，从 20 °C 到 25 °C 的温度范围内，葡萄糖在决定期[归一化强度（AU）从 7.58 增加到 9.62]和分化期（AU 从 5.85 增加到 6.41）增加。从 20 °C 到 30 °C ，松柏醛在无性期（5.79 到 6.83 AU）和分化期（6.66 到 7.40 AU）有所增加。在光周期（平均范围 = 0-122.91）和盐度（SD 范围 = 1.86-57.04）条件下，分化阶段第 9 区（肌酐和半胱氨酸）的平均值最高（22.97），SD 值最高（24.73）。在糖类、氨基酸和芳香化合物类别下，共鉴定出 30 种代谢物。本研究有助于了解生殖过程中细胞分化的内在机制。除了有助于控制商业化种植的育苗准备工作以及管理快速绿潮的形成外，本研究结果还可作为今后研究的重要参考点。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.