Dietary inclusion of Withania somnifera and Asparagus racemosus induces growth, activities of digestive enzymes, and transcriptional modulation of MyoD, MyoG, Myf5, and MRF4 genes in fish, Channa punctatus

IF 1.9 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sunil P. Trivedi , Shikha Dwivedi , Abha Trivedi , Adeel Ahmad Khan , Shefalee Singh , Kamlesh K. Yadav , Vivek Kumar , Shraddha Dwivedi , Vidyanand Tiwari , Yashika Awasthi
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引用次数: 0

Abstract

The present study explores growth potential of two medicinal herbs, Withania somnifera (Ashwagandha or ‘A') and Asparagus racemosus (Shatavari or ‘S') after their dietary inclusion in fish, Channa punctatus (13.5 ± 2 g; 11.5 ± 1 cm). Three hundred well-acclimatized fish were distributed into 10 groups- C (Control), S1 (1% S), S2 (2% S), S3 (3% S), A1 (1% A), A2 (2% A), A3 (3% A), AS1 (1% A and S), AS2 (2% A and S), and AS3 (3% A and S), each having 10 specimens. Fish were fed with these diets for 60 days. The study was performed in triplicate. Growth indices- weight gain (WG), specific growth rate percentage (SGR%), feed intake (FI), and condition factor (CF), after 30 and 60 days, were found significantly (p < 0.05) up-regulated in all the groups, except S1, when compared to the C. A significant (p < 0.05) increase in final body weight (FBW) was noticed in all the groups, except S1, after 60 days. Relative to the control group, activities of lipase and amylase in the gut tissue were elevated in all groups, at both sampling times, with the exception of lipase in S1 at 60 days, and amylase in S1 at day 30 and day 60 and S2 at day 60. The mRNA expression of myogenic regulatory factors (MRFs) was also found to be significantly (p < 0.05) up-regulated with the highest fold changes recorded in AS3 for myoD (3.93 ± 0.91); myoG (6.71 ± 0.30); myf5 (4.40 ± 0.33); MRF4 (4.94 ± 0.21) in comparison to the C.

Abstract Image

Abstract Image

膳食中添加睡茄和天门冬可促进鱼类的生长、消化酶活性以及 MyoD、MyoG、Myf5 和 MRF4 基因的转录调节。
本研究探讨了两种药草--睡茄(Ashwagandha 或 'A')和天门冬(Shatavari 或 'S')--在加入鱼类鳢鱼(13.5 ± 2 克;11.5 ± 1 厘米)的膳食后的生长潜力。将 300 尾适应良好的鱼类分为 10 组--C(对照组)、S1、S2、S3、A1、A2、A3、AS1、AS2 和 AS3,每组 10 尾。鱼类在 60 天的喂养试验中,分别和混合喂养实验饲料。研究以三重复的方式进行。研究发现,30 天和 60 天后,鱼类的生长指数--增重(WG)、特定生长率(SGR)、摄食量(FI)和体况系数(CF)--均有显著差异(p<0.05)。
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来源期刊
CiteScore
4.60
自引率
4.50%
发文量
77
审稿时长
22 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part B: Biochemical and Molecular Biology (CBPB), focuses on biochemical physiology, primarily bioenergetics/energy metabolism, cell biology, cellular stress responses, enzymology, intermediary metabolism, macromolecular structure and function, gene regulation, evolutionary genetics. Most studies focus on biochemical or molecular analyses that have clear ramifications for physiological processes.
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