Antidiabetic and antioxidant potential of Crocin in high-fat diet plus streptozotocin-induced type-2 diabetic rats.

IF 3.5 3区 医学
Syed Mohammed Basheeruddin Asdaq, Basheerahmed Abdulaziz Mannasaheb, Raha Orfali, Ibrahim Ahmed Shaikh, Ahmed Alshehri, Adel Alghamdi, Meshal Mohammed Alrashdi, Moneer E Almadani, Faisal Mohammad Ali Abdalla
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Abstract

Objectives: Crocin, the principal water-soluble active constituent of saffron, possesses numerous pharmacological activities. The present investigation examined the potential antidiabetic and antioxidant characteristics of Crocin in rats with type-2 diabetes by administering it orally and intraperitoneally (i.p.).

Methods: After 2 weeks of a high-fat diet, streptozotocin (STZ) (i.p., 40 mg/kg) was administered to male adult rats to induce type-2 diabetes mellitus. Body weight and fasting blood glucose (FBG) were measured on days zero, weeks 1, and 2. At the end of 2 weeks of drug administration in their respective groups, fasting insulin and glucose levels were estimated, and insulin resistance (HOMA-IR) was determined. Intraperitoneal glucose (IPGTT) and insulin tolerance tests (ITT) were carried out. Histopathological investigation and biochemical parameters were estimated in pancreatic tissues.

Results: The Crocin (100 mg/kg) treatment has significantly improved body weight, abatement of FBG, fasting insulin, and HOMA-IR. Likewise, Crocin treatment significantly improved the glucose and insulin challenges. We observed a significantly marked elevation in endogenous antioxidant enzymes in Crocin-treated groups. Similarly, Crocin treatment reversed the histopathological changes and restored the normal integrity and function of the pancreas.

Conclusion: The overall finding indicates that intraperitoneal administration of Crocin demonstrated better control of glycemic level and body weight. Further, it has improved insulin levels in the serum and potentiated antioxidant properties.

克罗霉素在高脂饮食加链脲佐菌素诱导的 2 型糖尿病大鼠中的抗糖尿病和抗氧化潜力
目的:藏红花的主要水溶性活性成分藏红花苷具有多种药理活性。本研究通过口服和腹腔注射藏红花苷对 2 型糖尿病大鼠进行研究,探讨藏红花苷潜在的抗糖尿病和抗氧化特性:方法:雄性成年大鼠经2周高脂肪饮食后,给予链脲佐菌素(STZ)(40 mg/kg)诱发2型糖尿病。在第 0 天、第 1 周和第 2 周测量体重和空腹血糖 (FBG)。在各组给药 2 周结束时,估算空腹胰岛素和葡萄糖水平,并测定胰岛素抵抗(HOMA-IR)。还进行了腹腔内葡萄糖(IPGTT)和胰岛素耐受试验(ITT)。对胰腺组织进行了组织病理学检查和生化指标评估:结果:克罗霉素(100 毫克/千克)治疗明显改善了体重,降低了 FBG、空腹胰岛素和 HOMA-IR。同样,克罗霉素治疗也明显改善了葡萄糖和胰岛素挑战。我们观察到,克罗霉素治疗组的内源性抗氧化酶明显升高。同样,克罗霉素治疗逆转了组织病理学变化,恢复了胰腺的正常完整性和功能:总体研究结果表明,腹腔注射克罗霉素能更好地控制血糖水平和体重。此外,它还改善了血清中的胰岛素水平,并增强了抗氧化特性。
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来源期刊
International Journal of Immunopathology and Pharmacology
International Journal of Immunopathology and Pharmacology Immunology and Microbiology-Immunology
自引率
0.00%
发文量
88
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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