Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Seyedeh-Sara Hashemi, Ali-Akbar Mohammadi, Mehdi Kian, Alireza Rafati, Mojtaba Ghaedi, Behzad Ghafari
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引用次数: 0

Abstract

Objectives: In the present study, we evaluated the effect of a nanofibrous scaffold including polycaprolactone (PCL), chitosan (CHT), and bentonite nanoparticles (Ben-NPS) on wound healing in order to introduce a novel dressing for burn wounds.

Materials and methods: PCL, PCL/CHT, and PCL/CHT/Ben-NPS nanofibrous scaffolds were fabricated by the electrospinning technique. Their structural and physiochemical characteristics were investigated by Fourier-transform infrared spectroscopy (FTIR) analysis, scanning electron microscopy (SEM), tensile strength, water contact angle, as well as, swelling and degradation profiles test. The disc diffusion assay was carried out to investigate the antibacterial potential of the scaffolds. In addition, the cell viability and proliferation ability of human dermal fibroblasts (HDFs) on the scaffolds were assessed using MTT assay as well as SEM imaging. The wound-healing property of the nanofibrous scaffolds was evaluated by histopathological investigations during 3,7, and 14 days in a rat model of burn wounds.

Results: SEM showed that all scaffolds had three-dimensional, beadles-integrated structures. Adding Ben-NPS into the PCL/CHT polymeric composite significantly enhanced the mechanical, swelling, and antibacterial properties. HDFs had the most cell viability and proliferation values on the PCL/CHT/Ben-NPS scaffold. Histopathological evaluation in the rat model revealed that dressing animal wounds with the PCL/CHT/Ben-NPS scaffold promotes wound healing.

Conclusion: The PCL/CHT/Ben-NPS scaffold has promising regenerative properties for accelerating skin wound healing.

含有膨润土纳米颗粒的聚己内酯/壳聚糖纳米纤维支架的制备及其在深二度烧伤大鼠模型中再生效果的评估。
研究目的在本研究中,我们评估了包括聚己内酯(PCL)、壳聚糖(CHT)和膨润土纳米颗粒(Ben-NPS)在内的纳米纤维支架对伤口愈合的影响,以便为烧伤伤口引入一种新型敷料:采用电纺丝技术制备 PCL、PCL/CHT 和 PCL/CHT/Ben-NPS 纳米纤维支架。通过傅立叶变换红外光谱(FTIR)分析、扫描电子显微镜(SEM)、拉伸强度、水接触角以及膨胀和降解曲线测试研究了它们的结构和理化特性。为研究支架的抗菌潜力,还进行了盘扩散试验。此外,还利用 MTT 试验和扫描电镜成像评估了支架上人真皮成纤维细胞(HDFs)的细胞活力和增殖能力。在大鼠烧伤模型中,通过 3、7 和 14 天的组织病理学调查评估了纳米纤维支架的伤口愈合性能:结果:扫描电子显微镜显示,所有支架都具有三维珠状一体化结构。在 PCL/CHT 聚合物中添加 Ben-NPS 能显著增强其机械、膨胀和抗菌性能。在 PCL/CHT/Ben-NPS 支架上,HDF 的细胞活力和增殖值最高。大鼠模型的组织病理学评估显示,用 PCL/CHT/Ben-NPS 支架包扎动物伤口可促进伤口愈合:结论:PCL/CHT/Ben-NPS 支架在加速皮肤伤口愈合方面具有良好的再生特性。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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