Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans-cleavage with MnO2 nanosheets†

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-02-15 DOI:10.1039/d3cc06186h

Renpeng Xia , Nan Ouyang , Tingting Wang , Yuan Zhuang , Peng Miao

引用次数: 0

Abstract

The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO₂ nanosheets. By monitoring the trans-cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.

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基于加速 CRISPR/Cas12a 与 MnO2 纳米片反式裂解的谷胱甘肽电化学检测技术

谷胱甘肽介导的 MnO2 纳米片还原加速了 CRISPR/Cas12a 系统。通过监测 DNA 探针的反式裂解事件，建立了谷胱甘肽检测的电化学方法，其检测限为 3.5 pM。它为血浆分析提供了一种性能令人满意的工具，表明谷胱甘肽检测法具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.