Observation of a Bone Invasion Model of Aspergillus fumigatus In Vitro and Analysis of the Antifungal Susceptibility.

IF 3.6 3区 生物学 Q2 MYCOLOGY
Yan Shao, Min Shen, Dan Peng, Wenxu Cheng, Chenggang Mao, Heng Zhang, Yi Sun
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Abstract

Background: Recently, the prevalence of invasive fungal infections has been on the rise, and one of the prevalent symptoms frequently observed is bone deterioration and bone loss.

Materials and methods: Using an in vitro model we studied how Aspergillus fumigatus invades the bone. Pathological analysis was then employed to observe the structure and distinctive features of the invading fungal elements within the bone invasion model. Meanwhile, the antifungal effects of itraconazole, voriconazole, posaconazole, and amphotericin B were evaluated.

Results: The pathological findings showed that in the experimental group, fungal spores and hyphae invaded the bone tissue or were observed growing in the vicinity of the bone edge tissues, as indicated by both HE and PAS staining. In contrast, no fungal elements were observed in the control group, indicating that the in vitro bone invasion model of A. fumigatus was successfully constructed. Furthermore, the findings from the antifungal sensitivity test demonstrated that the lowest effective concentrations of antifungal drugs against the bone invasion model were as follows: 4 μg/ml for itraconazole, 0.5 μg/ml for voriconazole, 2 μg/ml for posaconazole, and 2 μg/ml for amphotericin B.

Discussion: The successful construction of the bone invasion model of A. fumigatus has provided a solid basis for future investigations into the mechanisms underlying A. fumigatus bone invasion and the study of its virulence factors. Utilizing bone models is of utmost importance in advancing the development of novel antifungal treatment approaches, as well as in effectively preventing and treating fungal bone invasion and osteolytic diseases.

Abstract Image

体外曲霉菌骨侵袭模型观察及抗真菌敏感性分析
背景:近来,侵袭性真菌感染的发病率呈上升趋势,经常观察到的普遍症状之一是骨质退化和骨质流失:我们利用体外模型研究了烟曲霉如何侵入骨骼。材料:我们利用体外模型研究了曲霉菌是如何侵入骨骼的,然后利用病理分析观察了侵入骨骼模型的真菌成分的结构和特征。同时,对伊曲康唑、伏立康唑、泊沙康唑和两性霉素 B 的抗真菌效果进行了评估:病理结果显示,实验组的 HE 染色和 PAS 染色显示,真菌孢子和菌丝侵入骨组织或在骨边缘组织附近生长。相比之下,对照组未观察到任何真菌元素,这表明成功构建了烟曲霉体外骨侵袭模型。此外,抗真菌药敏试验结果表明,抗真菌药物对骨侵袭模型的最低有效浓度如下:伊曲康唑为 4 μg/ml,伏立康唑为 0.5 μg/ml,泊沙康唑为 2 μg/ml,两性霉素 B 为 2 μg/ml:烟曲霉骨侵袭模型的成功构建为今后研究烟曲霉骨侵袭的机制及其毒力因子提供了坚实的基础。利用骨模型对开发新型抗真菌治疗方法以及有效预防和治疗真菌骨侵袭和溶骨性疾病具有极其重要的意义。
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来源期刊
Mycopathologia
Mycopathologia 生物-真菌学
CiteScore
6.80
自引率
3.60%
发文量
76
审稿时长
3 months
期刊介绍: Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.
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