Structural biology of SARS-CoV-2

3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology
Asiya Kamber Zaidi, Sunny Dawoodi
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引用次数: 0

Abstract

This chapter provides a comprehensive overview of the techniques employed to unravel the structural biology of SARS-CoV-2, facilitating a deeper understanding of the virus for developing future therapeutic strategies. Various techniques such as Electron microscopy (EM) for capturing high-resolution images of the virus and X-ray crystallography used for determining atomic-level structures of viral proteins are discussed. Cryo-electron microscopy (cryo-EM) imaging is also examined as a powerful tool for visualizing the virus's structure in its native state. Intracellular detection and tracking of SARS-CoV-2 are discussed, highlighting the techniques employed to study the virus's behavior within host cells. The chapter further explores how cryo-EM has been instrumental in delivering high-quality structural information on SARS-CoV-2, enabling researchers to better understand its mechanisms of infection and replication. The structural visualization of SARS-CoV-2 is then presented, focusing on key components such as the spike protein structure, RNA polymerase structure, and the visualization of intact and in-situ virions using cryo-electron tomography (cryo-ET). Lastly, the chapter touches upon the application of nuclear magnetic resonance (NMR) spectroscopy for studying the dynamics and interactions of viral proteins

SARS-CoV-2 的结构生物学
本章全面概述了用于揭示 SARS-CoV-2 结构生物学的各种技术,这些技术有助于加深对病毒的了解,从而制定未来的治疗策略。本文讨论了各种技术,如用于捕捉病毒高分辨率图像的电子显微镜(EM)和用于确定病毒蛋白质原子级结构的 X 射线晶体学。冷冻电子显微镜(cryo-EM)成像技术作为一种强大的工具,可用于观察病毒在原生状态下的结构,本文也对其进行了研究。本章讨论了 SARS-CoV-2 的细胞内检测和跟踪,重点介绍了研究病毒在宿主细胞内行为的技术。本章进一步探讨了低温电子显微镜如何有助于提供有关 SARS-CoV-2 的高质量结构信息,使研究人员能够更好地了解其感染和复制机制。然后介绍了 SARS-CoV-2 的结构可视化,重点是尖峰蛋白结构、RNA 聚合酶结构等关键部分,以及利用低温电子断层扫描(cryo-ET)对完整病毒和原位病毒的可视化。最后,本章介绍了核磁共振(NMR)光谱在研究病毒蛋白质动态和相互作用方面的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Progress in Molecular Biology and Translational Science (PMBTS) provides in-depth reviews on topics of exceptional scientific importance. If today you read an Article or Letter in Nature or a Research Article or Report in Science reporting findings of exceptional importance, you likely will find comprehensive coverage of that research area in a future PMBTS volume.
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