LC-MS bioanalysis of protein biomarkers and protein therapeutics in formalin-fixed paraffin-embedded tissue specimens.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-02-01 Epub Date: 2024-01-16 DOI:10.4155/bio-2023-0210

Mashanipalya G Jagadeeshaprasad, Jianing Zeng, Naiyu Zheng

引用次数: 0

Abstract

Formalin-fixed paraffin-embedded (FFPE) is a form of preservation and preparation for biopsy specimens. FFPE tissue specimens are readily available as part of oncology studies because they are often collected for disease diagnosis or confirmation. FFPE tissue specimens could be extremely useful for retrospective studies on protein biomarkers because the samples preserved in FFPE blocks could be stable for decades. However, LC-MS bioanalysis of FFPE tissues poses significant challenges. In this Perspective, we review the benefits and recent developments in LC-MS approach for targeted protein biomarker and protein therapeutic analysis using FFPE tissues and their clinical and translational applications. We believe that LC-MS bioanalysis of protein biomarkers in FFPE tissue specimens represents a great potential for its clinical applications.

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对福尔马林固定石蜡包埋组织标本中的蛋白质生物标记物和蛋白质疗法进行液相色谱-质谱生物分析。

福尔马林固定石蜡包埋（FFPE）是活检标本的一种保存和制备方式。FFPE 组织标本在肿瘤学研究中很容易获得，因为它们通常是为疾病诊断或确诊而收集的。FFPE 组织标本对于蛋白质生物标记物的回顾性研究非常有用，因为保存在 FFPE 块中的样本可以稳定数十年。然而，对 FFPE 组织进行 LC-MS 生物分析是一项重大挑战。在本视角中，我们回顾了利用 FFPE 组织进行靶向蛋白质生物标记物和蛋白质治疗分析的 LC-MS 方法的优势和最新进展，以及它们在临床和转化方面的应用。我们相信，LC-MS 生物分析 FFPE 组织标本中的蛋白质生物标记物具有巨大的临床应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.