GPC2 as a diagnostic and prognostic marker regulated progression of colorectal cancer

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology Pub Date : 2024-02-01 DOI:10.1016/j.ajg.2023.11.006

Rugang Wang , Xuan Lin

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引用次数: 0

Abstract

Background and study aims

Glypican 2 (GPC2) is a member of the glypican gene family and is expressed in multiple kinds of cancer. However, the function and mechanism of GPC2 in colorectal cancer remains unclear. In this study, we aimed to identify the role of GPC2 on tumor cell proliferation and survival in colorectal cancer.

Patients and methods

Ten pairs of colon cancer and matched normal colon tissues were collected in this research. GEPIA was used to analysis the GPC2 gene expression profile in TGCA data base. RT-qPCR and western blot assay were performed to determine the mRNA and protein expressions. CCK-8, Flow cytometry and colon formation assay were applied to evaluate cell viability. IHC staining was performed to evaluate the protein expression in tissues. The function of GPC2 in vivo was verified by an animal model of colon cancer.

Results

Through the bioinformatics analysis and qRT-PCR validation, we found that GPC2 was upregulated in the colon cancer tissues and cells. GPC2 knockdown suppressed cell proliferation in vitro and in vivo was confirmed by the results of CCK-8, colony formation assays, and tumor xenograft models. Moreover, by the analysis of flow cytometry assay and gain-or-loss function experiments, we discovered that CEP164 was highly associated with the expression state of GPC2, and mediated G2/M-phase arrest in GPC2-downregulated tumor cells.

Conclusion

GPC2 might be a novel oncogenic gene in colorectal cancer, suggesting that it could be a considerable marker for the diagnosis and prognosis of colorectal cancer.

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GPC2 作为调节结直肠癌进展的诊断和预后标志物。

背景和研究目的：糖蛋白 2（GPC2）是糖蛋白基因家族的成员，在多种癌症中均有表达。然而，GPC2 在结直肠癌中的功能和机制仍不清楚。在这项研究中，我们旨在确定 GPC2 在结直肠癌中对肿瘤细胞增殖和存活的作用：本研究收集了 10 对结肠癌和匹配的正常结肠组织。采用 GEPIA 分析 TGCA 数据库中的 GPC2 基因表达谱。采用 RT-qPCR 和 Western 印迹法测定 mRNA 和蛋白质的表达。应用 CCK-8、流式细胞术和结肠形成试验评估细胞活力。通过 IHC 染色评估组织中的蛋白表达。通过结肠癌动物模型验证了 GPC2 在体内的功能：结果：通过生物信息学分析和 qRT-PCR 验证，我们发现 GPC2 在结肠癌组织和细胞中上调。CCK-8、集落形成试验和肿瘤异种移植模型的结果证实，GPC2 基因敲除抑制了体外和体内的细胞增殖。此外，通过流式细胞术分析和功能增益或丧失实验，我们发现 CEP164 与 GPC2 的表达状态高度相关，并在 GPC2 下调的肿瘤细胞中介导 G2/M 期停滞：结论：GPC2可能是结直肠癌中的一个新的致癌基因，它可能是诊断和预后结直肠癌的一个重要标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arab Journal of Gastroenterology Medicine-Gastroenterology

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.