In vitro chemical treatment of silk increases the expression of pro-inflammatory factors and facilitates degradation in rats.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Jinfeng Lv, Jieping Wang, Yao Zeng, Simeng Tian, Fei Wang, Yixue Zhai, Qian Zhou, Xiyue Luo, Xuanjie Zhang, Bin Liu, Chan Zhou
{"title":"In vitro chemical treatment of silk increases the expression of pro-inflammatory factors and facilitates degradation in rats.","authors":"Jinfeng Lv, Jieping Wang, Yao Zeng, Simeng Tian, Fei Wang, Yixue Zhai, Qian Zhou, Xiyue Luo, Xuanjie Zhang, Bin Liu, Chan Zhou","doi":"10.1177/22808000231222704","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Silk fiber is difficult to degrade in vivo, which limits its application in tissue engineering materials such as artificial nerves. Therefore, in this study aim to promote its degradation in vivo by chemical treating silk fibers in vitro.</p><p><strong>Materials and methods: </strong>Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) observations, mechanical test, Fourier transform infrared spectroscopy (FT-IR) measurements were used to investigate the degradation effect of chemicals (hydrochloric acid, phosphoric acid, acetic acid, sodium hydroxide, calcium hydroxide, sodium bicarbonate, and calcium chloride) on silk fiber in vitro. Immunofluorescence staining and transcriptome analysis were used to investigate the effect of inflammatory factors on the degradation of chemically treated silk fiber in rats.</p><p><strong>Results: </strong>(1) Silks were separated into finer fibers in each group. (2) FT-IR absorption peaks of amides I, II, and III overlap in each group. (3) Silk degradation degree in each group was higher than that in an untreated group. The calcium chloride-treated group was completely degraded. (4) Fibronectin, collagen I, collagen III, integrin α and CD68 were immunofluorescence positive in all vegetation section. (5) There were no significant differences in the expressions of collagen I, collagen III, and fibronectin in the vegetations formed on the 14th day of subcutaneous implantation, while integrin α, CD68, TNF-α, IL-1b, and IL-23 express at higher levels with IL-10 at lower levels.</p><p><strong>Conclusions: </strong>All chemicals could completely degrade silk; however, their degradation products were not the same. The chemicals change the mechanical properties of silk by separating it into finer fibers, which increase the contact surface area between the silk and tissue fluid, accelerating the degradation of monofilaments in vivo by promoting inflammation and macrophage activity through the increased and decreased expressions of pro- and anti-inflammatory factors, respectively.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000231222704"},"PeriodicalIF":3.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biomaterials & Functional Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/22808000231222704","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives: Silk fiber is difficult to degrade in vivo, which limits its application in tissue engineering materials such as artificial nerves. Therefore, in this study aim to promote its degradation in vivo by chemical treating silk fibers in vitro.

Materials and methods: Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) observations, mechanical test, Fourier transform infrared spectroscopy (FT-IR) measurements were used to investigate the degradation effect of chemicals (hydrochloric acid, phosphoric acid, acetic acid, sodium hydroxide, calcium hydroxide, sodium bicarbonate, and calcium chloride) on silk fiber in vitro. Immunofluorescence staining and transcriptome analysis were used to investigate the effect of inflammatory factors on the degradation of chemically treated silk fiber in rats.

Results: (1) Silks were separated into finer fibers in each group. (2) FT-IR absorption peaks of amides I, II, and III overlap in each group. (3) Silk degradation degree in each group was higher than that in an untreated group. The calcium chloride-treated group was completely degraded. (4) Fibronectin, collagen I, collagen III, integrin α and CD68 were immunofluorescence positive in all vegetation section. (5) There were no significant differences in the expressions of collagen I, collagen III, and fibronectin in the vegetations formed on the 14th day of subcutaneous implantation, while integrin α, CD68, TNF-α, IL-1b, and IL-23 express at higher levels with IL-10 at lower levels.

Conclusions: All chemicals could completely degrade silk; however, their degradation products were not the same. The chemicals change the mechanical properties of silk by separating it into finer fibers, which increase the contact surface area between the silk and tissue fluid, accelerating the degradation of monofilaments in vivo by promoting inflammation and macrophage activity through the increased and decreased expressions of pro- and anti-inflammatory factors, respectively.

对蚕丝进行体外化学处理会增加促炎因子的表达,并促进大鼠体内蚕丝的降解。
目的:蚕丝纤维很难在体内降解,这限制了其在人工神经等组织工程材料中的应用。因此,本研究旨在通过在体外对蚕丝纤维进行化学处理,促进其在体内降解:采用硫酸盐-聚丙烯酰胺凝胶电泳(SDS-PAGE)、扫描电子显微镜(SEM)观察、力学测试、傅立叶变换红外光谱(FT-IR)测量等方法研究化学试剂(盐酸、磷酸、醋酸、氢氧化钠、氢氧化钙、碳酸氢钠和氯化钙)对体外蚕丝纤维的降解作用。免疫荧光染色和转录组分析用于研究炎症因子对大鼠化学处理丝纤维降解的影响。(2)各组酰胺 I、II 和 III 的傅立叶变换红外吸收峰重叠。(3)各组的蚕丝降解程度均高于未处理组。氯化钙处理组完全降解。(4)所有植被切片中的纤连蛋白、胶原 I、胶原 III、整合素 α 和 CD68 均呈免疫荧光阳性。(5)在皮下植入第 14 天形成的植被中,胶原 I、胶原 III 和纤连蛋白的表达无显著差异,而整合素 α、CD68、TNF-α、IL-1b 和 IL-23 的表达水平较高,IL-10 的表达水平较低:结论:所有化学物质都能完全降解蚕丝,但它们的降解产物却不尽相同。化学物质通过将蚕丝分离成更细的纤维改变了蚕丝的机械性能,从而增加了蚕丝与组织液的接触表面积,通过分别增加和减少促炎因子和抗炎因子的表达来促进炎症和巨噬细胞的活性,加速了单丝在体内的降解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信