A single mutation in the mosquito (Aedes aegypti) olfactory receptor 8 causes loss of function to 1-octen-3-ol

IF 3.2 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology Pub Date : 2024-01-12 DOI:10.1016/j.ibmb.2023.104069

Olga Frunze, Dain Lee, Seungha Lee, Hyung Wook Kwon

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Abstract

The host-seeking behavior of mosquitoes have long been established to be primarily odor-mediated. In this process, olfactory receptors (Ors) play a critical role. 1-Octen-3-ol is a common volatile compound that is attractive to hematophagous arthropods such as mosquitos. The olfactory receptor 8 (AaOr8) on the tip of the stylet and maxillary palp of Aedes aegypti is tuned to 1-octen-3-ol, which is required for mosquitoes to quickly find blood vessels from a vertebrate host. However, little is known about the interaction of AaOr8 with 1-octen-3-ol which was studied in vivo and in silico in this study. The molecular binding poses and energies between ligands and the receptor were investigated. Three mutants of AaOr8 were cloned and compared with in vivo calcium imaging utilizing heterologous expression systems. As a result, our findings imply that a genetic disruption including targeted modification of Ors genes may be used to reduce mosquito bites.

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蚊子（埃及伊蚊）嗅觉受体 8 的单一突变会导致 1-辛烯-3-醇功能丧失。

长期以来，蚊子寻找宿主的行为主要是由气味介导的。在这一过程中，嗅觉受体（Ors）起着至关重要的作用。1 辛烯-3-醇是一种常见的挥发性化合物，对蚊子等食血节肢动物具有吸引力。埃及伊蚊花柱和上颚顶端的嗅觉受体 8（AaOr8）对 1-辛烯-3-醇具有调节作用，蚊子需要它来迅速找到脊椎动物宿主的血管。然而，人们对 AaOr8 与 1-辛烯-3-醇的相互作用知之甚少。本研究对配体与受体之间的分子结合位置和能量进行了研究。克隆了三种 AaOr8 突变体，并利用异源表达系统与体内钙成像进行了比较。因此，我们的研究结果表明，可以利用基因干扰（包括有针对性地修改 Ors 基因）来减少蚊虫叮咬。

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来源期刊

Insect Biochemistry and Molecular Biology 生物-昆虫学

CiteScore

7.40

自引率

5.30%

发文量

105

审稿时长

40 days

期刊介绍： This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.