Taimoor Khan, Muhammad Uzair Javed, Tehreem Mahmood, Bushra Khan, Tariq Khan, Muhammad Asad Ullah, Razia Khurshid, Gouhar Zaman, Christophe Hano, Nathalie Giglioli-Guivarc’h, Bilal Haider Abbasi
{"title":"Enhancement in the production of phenolic compounds from Fagonia indica callus cultures via Fusarium oxysporum triggered elicitation","authors":"Taimoor Khan, Muhammad Uzair Javed, Tehreem Mahmood, Bushra Khan, Tariq Khan, Muhammad Asad Ullah, Razia Khurshid, Gouhar Zaman, Christophe Hano, Nathalie Giglioli-Guivarc’h, Bilal Haider Abbasi","doi":"10.1007/s11627-023-10358-0","DOIUrl":null,"url":null,"abstract":"<p><i>Fagonia indica</i> Burm.f. <i>(1768)</i> is a medicinally important plant showing diverse pharmaceutical benefits. It is renowned for its ability to biosynthesize several anticancer and anti-inflammatory metabolites. For the eco-friendly and sustainable synthesis of phytochemicals and plant biomass, a biotechnological technique, “elicitation,” is a highly effective method in various <i>in vitro</i> cultures. The present study includes using various concentrations of <i>Fusarium oxysporum</i> Schlecht. as an elicitor in callus cultures of <i>Fagonia indica</i>. The main goal was to achieve enhancement in biomass production and secondary metabolism. The findings demonstrated that maximum biomass production (FW: 167.42 ± 3.99 g per 100 mL; DW: 12.53 ± 1.04 g per 100 mL) was observed at 50 mg L<sup>−1</sup> of <i>Fusarium oxysporum</i> as compared to the control. Secondary metabolites showed immense production (phenolic content (9.68 ± 0.23 µg mg<sup>−1</sup>); flavonoid content (2.814808 ± 0.11 µg mg<sup>−1</sup>)) in callus cultures treated with 10 mg L<sup>−1</sup> of <i>Fusarium oxysporum</i> as compared with control. Moreover, the cultures possessed the highest antioxidant capacity, as determined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS<sup>•+</sup>) radical cation based assay and α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging assay, ((821.51 ± 3.20 µmol TEAC per mg DW of ABTS inhibition) (91% ± 1.45 of DPPH inhibition)) at 10 mg L<sup>−1</sup> concentration of <i>Fusarium oxysporum</i>, and the maximum ferric ion reducing activity (219.29 ± 2.36 µmol TEAC per mg DW) was noticed at 1.0 mg L<sup>−1</sup> concentration of <i>F. oxysporum</i>. <i>Fagonia indica</i> cultures also indicated the highest percent inhibition against cyclooxygenases (COX-1: 51.93% ± 1.74 and COX-2: 40.57% ± 1.99), lipoxygenase (15-LOX: 65.72% ± 1.44), and phospholipase A2 (sPLA2: 49.29% ± 1.75), when treated with different concentrations of <i>F. oxysporum</i>. HPLC analyses showed a significant accumulation of pharmacologically active components in the treated samples, with kaempferol (1245.56 mg g<sup>−1</sup>) and myricetin (1139.63 mg g<sup>−1</sup>) as the most accumulated compounds in the cultures with 10.0 mg L<sup>−1</sup> concentration of <i>Fusarium</i> in contrast to the control. These findings revealed that in callus cultures of <i>F. indica</i>, <i>F. oxysporum</i> could boost biomass accumulation and secondary metabolite production.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"3 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro Cellular & Developmental Biology - Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11627-023-10358-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Fagonia indica Burm.f. (1768) is a medicinally important plant showing diverse pharmaceutical benefits. It is renowned for its ability to biosynthesize several anticancer and anti-inflammatory metabolites. For the eco-friendly and sustainable synthesis of phytochemicals and plant biomass, a biotechnological technique, “elicitation,” is a highly effective method in various in vitro cultures. The present study includes using various concentrations of Fusarium oxysporum Schlecht. as an elicitor in callus cultures of Fagonia indica. The main goal was to achieve enhancement in biomass production and secondary metabolism. The findings demonstrated that maximum biomass production (FW: 167.42 ± 3.99 g per 100 mL; DW: 12.53 ± 1.04 g per 100 mL) was observed at 50 mg L−1 of Fusarium oxysporum as compared to the control. Secondary metabolites showed immense production (phenolic content (9.68 ± 0.23 µg mg−1); flavonoid content (2.814808 ± 0.11 µg mg−1)) in callus cultures treated with 10 mg L−1 of Fusarium oxysporum as compared with control. Moreover, the cultures possessed the highest antioxidant capacity, as determined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical cation based assay and α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging assay, ((821.51 ± 3.20 µmol TEAC per mg DW of ABTS inhibition) (91% ± 1.45 of DPPH inhibition)) at 10 mg L−1 concentration of Fusarium oxysporum, and the maximum ferric ion reducing activity (219.29 ± 2.36 µmol TEAC per mg DW) was noticed at 1.0 mg L−1 concentration of F. oxysporum. Fagonia indica cultures also indicated the highest percent inhibition against cyclooxygenases (COX-1: 51.93% ± 1.74 and COX-2: 40.57% ± 1.99), lipoxygenase (15-LOX: 65.72% ± 1.44), and phospholipase A2 (sPLA2: 49.29% ± 1.75), when treated with different concentrations of F. oxysporum. HPLC analyses showed a significant accumulation of pharmacologically active components in the treated samples, with kaempferol (1245.56 mg g−1) and myricetin (1139.63 mg g−1) as the most accumulated compounds in the cultures with 10.0 mg L−1 concentration of Fusarium in contrast to the control. These findings revealed that in callus cultures of F. indica, F. oxysporum could boost biomass accumulation and secondary metabolite production.
期刊介绍:
Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.