Tissue culture response and in vitro plant regeneration of ‘Haruka’ (Cerasus Sato-zakura Group ‘Haruka’), a new cultivar of Japanese flowering cherry

IF 2.2 3区生物学 Q4 CELL BIOLOGY

In Vitro Cellular & Developmental Biology - Plant Pub Date : 2024-01-12 DOI:10.1007/s11627-023-10407-8

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引用次数: 0

Abstract

This study describes the in vitro regeneration of ‘Haruka’ plants, a new cultivar of Japanese flowering cherry registered in 2021 by the Japanese statutory authority. As this is a double-flowered cultivar produced by inter-specific hybridization, in vitro regeneration is an effective method for large-scale propagation. To promote proliferation, apical shoots were cultured on half-strength Murashige and Skoog medium supplemented with 5 μM 6-benzylaminopurine. The highest average of 7.8 shoots per explant was obtained at 15°C in the dark. Cultures were maintained in continuous darkness for 12 wk and then transferred to lighting conditions under a 16-h photoperiod at 25°C. Subsequently, a 100% rooting rate was obtained with the application of 1 μM indole-3-butyric acid in combination with 0.1 μM naphthaleneacetic acid. Additionally, the number of roots per shoot and the maximum length of roots were significantly higher under exposure to pink light illumination provided by cold cathode fluorescent lamps emitting red- and blue-colored light at a ratio of 80% and 20%, respectively. More than 95% of the regenerated plantlets survived after ex vitro acclimatization.

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日本花樱新品种 "Haruka"（Cerasus Sato-zakura Group 'Haruka'）的组织培养反应和离体植株再生

摘要本研究描述了 "Haruka "植株的离体再生，这是 2021 年经日本法定机构注册的日本樱花新栽培品种。由于这是一个通过种间杂交产生的双花栽培品种，体外再生是大规模繁殖的有效方法。为促进增殖，顶端嫩枝在添加了 5 μM 6-苄基氨基嘌呤的半强度 Murashige 和 Skoog 培养基上进行培养。在 15°C 黑暗条件下，每个外植体平均获得 7.8 个嫩枝。培养物在连续黑暗条件下保持 12 周，然后转移到 25°C 光周期为 16 小时的光照条件下。随后，施用 1 μM 的吲哚-3-丁酸和 0.1 μM 的萘乙酸，生根率达到 100%。此外，在冷阴极荧光灯分别以 80% 和 20% 的比例发出红光和蓝光的粉红色光照下，每个芽的根数和根的最大长度都明显增加。经过离体适应后，超过 95% 的再生小植株存活下来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In Vitro Cellular & Developmental Biology - Plant 生物-发育生物学

CiteScore

5.00

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.