Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus

Nano Express Pub Date : 2024-01-10 DOI:10.1088/2632-959x/ad1d02

Nawras Osman, Calvin A. Omolo, M. A. Gafar, Nikita Devnarain, Sanjeev Rambharose, U. H. Ibrahim, V. Fasiku, Thiru Govender

{"title":"Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus","authors":"Nawras Osman, Calvin A. Omolo, M. A. Gafar, Nikita Devnarain, Sanjeev Rambharose, U. H. Ibrahim, V. Fasiku, Thiru Govender","doi":"10.1088/2632-959x/ad1d02","DOIUrl":null,"url":null,"abstract":"\n Surface functionalization of nanoparticles has shown potential in enhancing the efficacy of antibiotic-loaded nanosystems against drug-resistant bacteria. The objective of this study was to synthesize and characterize an acid-cleavable pH-responsive polymer from methoxy polyethylene glycol and oleylamine (mPEG-OA) to surface modify vancomycin (VCM)-loaded niosomes and to evaluate their antibacterial and anti-biofilm effectiveness against methicillin-resistant Staphylococcus aureus (MRSA). The novel mPEG-OA-coated niosomes were biocompatible, hemocompatible with size, polydispersity index, and zeta potential of 169.2 ± 1.6 nm, 0.21 ± 0.01 and -0.82 ± 0.22 mV, respectively. Under acidic conditions, mPEG-OA-coated niosomes exhibited a pH-responsive and sustained VCM release profile and in vitro antibacterial activity than non-coated niosomes and bare VCM. mPEG-OA-coated niosomes showed a significant reduction in biofilm formation at pH 6 compared to pH 7.4 (p= 0,0119). The in vivo efficacy of mPEG-OA-coated niosomes in the BALB/c mice skin infection model showed a 9.9-fold reduction in MRSA load compared to bare VCM. Histomorphologically, the mPEG-OA-coated niosomes group displayed the lowest bacterial load, tissue swelling, and inflammation. The results of this study demonstrate the potential of novel pH-responsive mPEG-OA-derived polymer coating to enhance bacterial killing kinetics, and antibacterial and anti-biofilm efficacies over conventional antibiotic and non-functionalized nano delivery systems.","PeriodicalId":118165,"journal":{"name":"Nano Express","volume":"70 3","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Express","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/2632-959x/ad1d02","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Surface functionalization of nanoparticles has shown potential in enhancing the efficacy of antibiotic-loaded nanosystems against drug-resistant bacteria. The objective of this study was to synthesize and characterize an acid-cleavable pH-responsive polymer from methoxy polyethylene glycol and oleylamine (mPEG-OA) to surface modify vancomycin (VCM)-loaded niosomes and to evaluate their antibacterial and anti-biofilm effectiveness against methicillin-resistant Staphylococcus aureus (MRSA). The novel mPEG-OA-coated niosomes were biocompatible, hemocompatible with size, polydispersity index, and zeta potential of 169.2 ± 1.6 nm, 0.21 ± 0.01 and -0.82 ± 0.22 mV, respectively. Under acidic conditions, mPEG-OA-coated niosomes exhibited a pH-responsive and sustained VCM release profile and in vitro antibacterial activity than non-coated niosomes and bare VCM. mPEG-OA-coated niosomes showed a significant reduction in biofilm formation at pH 6 compared to pH 7.4 (p= 0,0119). The in vivo efficacy of mPEG-OA-coated niosomes in the BALB/c mice skin infection model showed a 9.9-fold reduction in MRSA load compared to bare VCM. Histomorphologically, the mPEG-OA-coated niosomes group displayed the lowest bacterial load, tissue swelling, and inflammation. The results of this study demonstrate the potential of novel pH-responsive mPEG-OA-derived polymer coating to enhance bacterial killing kinetics, and antibacterial and anti-biofilm efficacies over conventional antibiotic and non-functionalized nano delivery systems.

查看原文本刊更多论文

用新型 pH 值响应涂层（mPEG-OA）修饰的 Niosomes 可增强万古霉素对耐甲氧西林金黄色葡萄球菌的抗菌和抗生物膜活性

纳米颗粒的表面功能化在提高抗生素纳米系统对抗药性细菌的疗效方面已显示出潜力。本研究的目的是合成并表征一种由甲氧基聚乙二醇和油胺（mPEG-OA）组成的可酸解的 pH 响应聚合物，用于表面修饰万古霉素（VCM）负载的niosomes，并评估其对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌和抗生物膜效果。新型 mPEG-OA 包覆的niosomes具有生物相容性和血液相容性，其尺寸、多分散指数和zeta电位分别为169.2 ± 1.6 nm、0.21 ± 0.01 和 -0.82 ± 0.22 mV。在酸性条件下，mPEG-OA 包覆的纳米囊泡比无包覆的纳米囊泡和裸露的 VCM 表现出对 pH 值的响应性和持续的 VCM 释放特性，以及体外抗菌活性。在 BALB/c 小鼠皮肤感染模型中，mPEG-OA 包裹层iosomes 的体内疗效显示，与裸 VCM 相比，MRSA 负荷减少了 9.9 倍。从组织形态学上看，mPEG-OA 包裹的药膜组的细菌量、组织肿胀和炎症程度最低。这项研究结果表明，与传统抗生素和非功能化纳米递送系统相比，新型 pH 响应 mPEG-OA 衍生聚合物涂层具有增强杀灭细菌动力学、抗菌和抗生物膜功效的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nano Express

CiteScore

6.40

自引率

0.00%

发文量