Quantification of Touch DNA on Glass, Plastic, and Ceramic Glasses

AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Pub Date : 2024-01-10 DOI:10.29165/ajarcde.v8i1.365

Novenalitha K. Sinukaban, I. K. Junitha, Ni Nyoman Wirasiti, Ni Suriani

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Abstract

In Indonesia, there are many criminal cases. Every criminal offender will certainly bring and leave something at the crime scene that can be used as a trial or evidence. In addition, perpetrators who accidentally or intentionally come into contact with surrounding objects can cause the transfer of trace evidence to these objects. DNA touch left on an object can provide information about the identity of individuals in contact with that object. This study aimed to determine the quantity and quality of DNA Touch in glasses made of glass, plastic, and ceramic that were touched by one proband and two probands. DNA extraction was carried out using the 5% Chelex method. using Univariate and one-way ANOVA. DNA quality was observed based on the comparison results of Å260 and Å280 on the SimpliNano spectrophotometer. The results showed that the concentration of DNA in glass, plastic and ceramics was not significantly different in each treatment. There was no interaction between the type of glass and hand touch, the average DNA concentration. Extracted DNA showed poor quality

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玻璃、塑料和陶瓷杯上触摸 DNA 的定量分析

在印度尼西亚，有许多刑事案件。每个刑事罪犯肯定会在犯罪现场携带和留下一些可用作审判或证据的东西。此外，罪犯无意或有意与周围物体接触，也会导致痕迹证据转移到这些物体上。遗留在物体上的 DNA 痕迹可以提供与该物体接触过的人的身份信息。本研究旨在确定由玻璃、塑料和陶瓷制成的玻璃杯中被一名原告和两名原告触摸过的 DNA Touch 的数量和质量。DNA 提取采用 5% Chelex 法。根据 SimpliNano 分光光度计上 Å260 和 Å280 的比较结果观察 DNA 质量。结果显示，玻璃、塑料和陶瓷中的 DNA 浓度在每种处理中都没有显著差异。玻璃和手的接触类型与平均 DNA 浓度之间没有交互作用。提取的 DNA 质量较差

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AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)

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