The influence of nutritional habits, body mass index and intestinal microbiota in mastocytosis on clinical symptoms using conventional culture and next generation sequencing

IF 4.6 2区医学 Q2 ALLERGY

Clinical and Translational Allergy Pub Date : 2024-01-13 DOI:10.1002/clt2.12310

Ewelina Harcęko-Zielińska, Marek Niedoszytko, Aleksandra Górska, Sylwia Małgorzewicz, Marta Gruchała-Niedoszytko, Marek Bronk, Slawomir Dąbrowski, Marta Chełminska, Ewa Jassem

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Abstract

Background

Mastocytosis is a rare neoplastic disease of the bone marrow associated with the proliferation and accumulation of mast cells in various internal organs, including the gastrointestinal tract. There are few studies describing the gut microbiome of patients with mastocytosis using next generation sequencing supported using traditional culture methods. The aims of the study were, firstly, the determination of nutrition habits, composition of the intestinal microflora and BMI in mastocytosis, and secondly, analysis of mastocytosis severity and symptoms depending on the composition of the intestinal microflora.

Methods

The study included 47 patients with indolent systemic mastocytosis and 18 healthy controls. All participants gave their informed consent to participate in the study. The study consisted of 3 parts: I-clinical assessment, II - examination of the intestinal microflora using the biochemical method, III - 16S rRNA sequencing.

Results

The nutrition habits and BMI of mastocytosis patients were similar to controls; however, most patients with mastocytosis had a low dietary vitamin and mineral content. As many as 94.5% of patients had too little fiber intake and mineral content. The most common cause of the abnormal stool test result with traditional culture was a titer of E. coli <10⁶. The low richness of microbiota species indicated by the Simpson index was observed in mastocytosis, p = 0.04. There were no significant differences in the composition of the intestinal microflora depending on the type of mastocytosis; however, the tryptase level correlated with the amount of Suterella, Barnesiellaceae, Eubacterium, Odoribacter, and Anaerostipes.

Conclusions

The nutritional habits and BMI of mastocytosis patients are similar to the general population, except for too little fiber intake and mineral content. The gastrointestinal symptoms of mastocytosis patients may be related to the low richness of microbiota species and the amount of Suterella, Barnesiellaceae, Eubacterium, Odoribacter, Anaerostipes, which correlated with tryptase levels.

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利用传统培养和新一代测序技术研究肥大细胞增多症患者的营养习惯、体重指数和肠道微生物群对临床症状的影响

背景肥大细胞增多症是一种罕见的骨髓肿瘤性疾病，与包括胃肠道在内的各种内脏器官中肥大细胞的增殖和积聚有关。在传统培养方法的支持下，利用新一代测序技术描述肥大细胞增多症患者肠道微生物组的研究很少。本研究的目的首先是确定肥大细胞增多症患者的营养习惯、肠道微生物菌群的组成和体重指数，其次是根据肠道微生物菌群的组成分析肥大细胞增多症的严重程度和症状。研究方法该研究包括 47 名患有系统性隐匿性肥大细胞增多症的患者和 18 名健康对照者。所有参与者均在知情的情况下同意参与研究。研究包括三个部分：I-临床评估，II-使用生化方法检查肠道微生物区系，III-16S rRNA 测序。结果乳腺增生症患者的营养习惯和体重指数与对照组相似；但大多数乳腺增生症患者的饮食中维生素和矿物质含量较低。多达 94.5% 的患者纤维摄入量和矿物质含量过低。传统培养的粪便检测结果异常的最常见原因是大肠杆菌滴度为 106。在肥大细胞增多症中，辛普森指数所显示的微生物群物种丰富度较低，p = 0.04。不同类型的乳腺增生症患者肠道微生物菌群的组成无明显差异；然而，胰蛋白酶水平与Suterella、Barnesiellaceae、Eubacterium、Odoribacter和Anaerostipes的数量相关。结论乳腺增生症患者的营养习惯和体重指数与普通人相似，只是纤维摄入量和矿物质含量太少。肥大细胞增多症患者的胃肠道症状可能与微生物区系物种丰富度低有关，与胰蛋白酶水平相关的苏特氏菌、巴氏杆菌科（Barnesiellaceae）、优博氏菌（Eubacterium）、奥多氏菌（Odoribacter）和厌氧菌（Anaerostipes）的数量也很低。

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来源期刊

Clinical and Translational Allergy Immunology and Microbiology-Immunology

CiteScore

7.50

自引率

4.50%

发文量

117

审稿时长

12 weeks

期刊介绍： Clinical and Translational Allergy, one of several journals in the portfolio of the European Academy of Allergy and Clinical Immunology, provides a platform for the dissemination of allergy research and reviews, as well as EAACI position papers, task force reports and guidelines, amongst an international scientific audience. Clinical and Translational Allergy accepts clinical and translational research in the following areas and other related topics: asthma, rhinitis, rhinosinusitis, drug hypersensitivity, allergic conjunctivitis, allergic skin diseases, atopic eczema, urticaria, angioedema, venom hypersensitivity, anaphylaxis, food allergy, immunotherapy, immune modulators and biologics, animal models of allergic disease, immune mechanisms, or any other topic related to allergic disease.