Effects of Salmonella Outer Membrane Vesicles on Intestinal Microbiota and Intestinal Barrier Function.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-04-01 Epub Date: 2024-01-12 DOI:10.1089/fpd.2023.0096

Meiying Luo, Suqian Li, Yang Yang, Junhang Sun, Yuman Su, Dechun Huang, Xin Feng, Huihua Zhang, Qien Qi

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引用次数: 0

Abstract

Salmonella enterica is one of the most important zoonotic pathogens causing foodborne gastroenteritis worldwide. Outer membrane vesicles (OMVs) are lipid-bilayer vesicles produced by Gram-negative bacteria, which contain biologically active components. We hypothesized that OMVs are an important weapon of S. enterica to initiate enteric diseases pathologies. In this study, the effects of S. enterica OMVs (SeOMVs) on intestinal microbiota and intestinal barrier function were investigated. In vitro fecal culture experiments showed that alpha diversity indexes and microbiota composition were altered by SeOMV supplementation. SeOMV supplementation showed an increase of pH, a decrease of OD630 and total short chain fatty acid (SCFA) concentrations. In vitro IPEC-J2 cells culture experiments showed that SeOMV supplementation did not affect the IPEC-J2 cell viability and the indicated genes expression. In vivo experiments in mice showed that SeOMVs had adverse effects on average daily gain (p < 0.05) and feed:gain ratio (p < 0.05), and had a tendency to decrease the final body weight (p = 0.073) in mice. SeOMV administration decreased serum interleukin-10 level (p < 0.05), decreased the relative abundance of bacteria belonging to the genera BacC-u-018 and Akkermansia (p < 0.05). Furthermore, SeOMV administration damaged the ileum mucosa (p < 0.05). These findings suggest that SeOMVs play an important role in the activation of intestinal inflammatory response induced by S. enterica, and downregulation of SCFA-producing bacteria is a possible mechanism.

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沙门氏菌外膜囊泡对肠道微生物群和肠屏障功能的影响

肠炎沙门氏菌是导致全球食源性肠胃炎的最重要的人畜共患病原体之一。外膜囊泡是革兰氏阴性细菌产生的脂质层囊泡，其中含有生物活性成分。我们推测，外膜囊泡是肠杆菌引发肠道疾病的重要武器。本研究调查了肠道病毒 OMVs（SeOMVs）对肠道微生物区系和肠道屏障功能的影响。体外粪便培养实验表明，补充 SeOMV 会改变阿尔法多样性指数和微生物群组成。补充 SeOMV 后，pH 值升高，OD630 和总短链脂肪酸（SCFA）浓度降低。体外 IPEC-J2 细胞培养实验表明，补充 SeOMV 不会影响 IPEC-J2 细胞的活力和相关基因的表达。小鼠体内实验表明，SeOMV 对小鼠的平均日增重有不利影响（p p = 0.073）。服用 SeOMV 会降低血清白细胞介素-10 水平（p BacC-u-018 和 Akkermansia）（p SeOMV 会损伤回肠粘膜（p SeOMVs 在肠炎球菌诱导的肠道炎症反应激活过程中发挥重要作用，下调 SCFA 产菌是可能的机制。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.