BLVRA exerts its biological effects to induce malignant properties of hepatocellular carcinoma cells via Wnt/β-catenin pathway

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Xinju Chen, Fangming Yang, Chuanlei Zhang, Xinting Wang, Changwei Yuan, Dandan Shi, Shuaishuai Zhu, Xiaotong Zhang, Xiaoqi Chen, Wenxia Zhao
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引用次数: 0

Abstract

The function of Biliverdin Reductase A (BLVRA) in hepatocellular carcinoma (HCC) cells proliferation, invasion and migration remains unclear. Therefore, this research intends to explore the effect of BLVRA on HCC cells growth and metastasis. BLVRA expression was analyzed in public dataset and examined by using western blot. The malignant function of BLVRA in HCC cell lines and its effect on Wnt/β-catenin pathway were measured. Analysis from GEPIA website showed that BLVRA expression was significantly increased in HCC tissues, and high expression of BLVRA resulted in worse prognosis of HCC patients. Results from western blot showed that BLVRA expression was obviously increased in HCC cell lines. Moreover, HepG2 and Hep3B cells in si-BLVRA-1 or si-BLVRA-2 group displayed an obvious reduction in its proliferation, cell cycle, invasion and migration compared to those in the si-control group. Additionally, si-BLVRA-1 or si-BLVRA-2 transfection significantly reduced the protein levels of Vimentin, Snail1 and Snail2, as well as decreased Bcl-2 expression and increased Bax and cleaved-caspase 3 expression. Furthermore, si-BLVRA treatment inhibited the protein levels of c-MYC, β-catenin, and Cyclin D1. After IWP-4 (Wnt/β-catenin inhibitor) treatment, the proliferation ability of HCC cells was significantly reduced. BLVRA expression was significantly increased in HCC tissues and cell lines, and knocked down of BLVRA could suppress the proliferation, invasion and migration in HCC cell lines, as well as induce cell apoptosis. Moreover, si-BLVRA transfection blocked the activation of Wnt/β-catenin pathway.

BLVRA通过Wnt/β-catenin通路发挥其生物效应,诱导肝癌细胞的恶性特性。
胆绿素还原酶A(BLVRA)在肝细胞癌(HCC)细胞增殖、侵袭和迁移中的功能尚不清楚。因此,本研究旨在探讨BLVRA对HCC细胞生长和转移的影响。在公共数据集中分析了BLVRA的表达,并通过Western印迹进行了检测。测定了BLVRA在HCC细胞系中的恶性功能及其对Wnt/β-catenin通路的影响。GEPIA网站的分析表明,BLVRA在HCC组织中的表达明显增加,BLVRA的高表达导致HCC患者的预后变差。Western印迹结果显示,BLVRA在HCC细胞系中的表达明显增加。此外,与 si-control 组相比,si-BLVRA-1 或 si-BLVRA-2 组的 HepG2 和 Hep3B 细胞在增殖、细胞周期、侵袭和迁移方面均明显降低。此外,si-BLVRA-1 或 si-BLVRA-2 转染显著降低了 Vimentin、Snail1 和 Snail2 的蛋白水平,降低了 Bcl-2 的表达,增加了 Bax 和裂解-天冬酶 3 的表达。此外,si-BLVRA 还能抑制 c-MYC、β-catenin 和 Cyclin D1 的蛋白水平。IWP-4(Wnt/β-catenin 抑制剂)处理后,HCC 细胞的增殖能力明显降低。BLVRA在HCC组织和细胞系中的表达明显增加,敲除BLVRA可抑制HCC细胞系的增殖、侵袭和迁移,并诱导细胞凋亡。此外,si-BLVRA转染可阻断Wnt/β-catenin通路的激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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