[Effect of L-carnitine and resveratrol complex on the profile of cytokines and regulatory proteins in normal and obese rats].

Q2 Medicine
Voprosy pitaniia Pub Date : 2023-01-01 Epub Date: 2023-10-30 DOI:10.33029/0042-8833-2023-92-6-83-97
V A Shipelin, N A Riger, A N Timonin, I V Gmoshinski, D B Nikityuk
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引用次数: 0

Abstract

Chronic inflammation in adipose tissue, peripheral organs and some compartments of the brain are among pathogenetic factors in obesity. The use of bioactive compounds with anti-inflammatory and hypolipidemic activity in the composition of specialized products and dietary supplements is considered as an approach in the diet therapy of obesity and related conditions. The aim of the research was to study the effect of a complex supplement containing resveratrol and L-carnitine (RC supplement) on the immunological parameters of inflammation (the profile of cytokines and regulatory proteins) in rats fed a balanced or hypercaloric diet. Material and methods. Male Wistar rats received for 63 days a standard balanced diet (SD) or a high-carbohydrate-high-fat diet (HFCD) with an excess of total fat and fructose, as well as RC supplement at a low (25 mg/kg body weight as Res and 300 mg/kg body weight as L-Car) or high (50 and 600 mg/kg body weight, respectively) dose. The content of leptin, ghrelin, cytokines and chemokines in blood serum (BS), lysates of white adipose tissue (WAT) and spleen, amygdala and hippocampus of the brain, the content of regulatory proteins Akt, IRS-1, GCK-3a/b, p70/S6, BAD, m-TOR, PTEN and S6 ribosomal protein in the amygdala and hippocampus have been studied the by multiplex immunoassay. Results. In rats that consumed RC as part of SD, there was a decrease in the levels of leptin and its ratio with ghrelin, the levels of proinflammatory cytokines (TNF-α, IL-1α, IL-12p40 and IL-12p70, IFN-γ) in BS and WAT, chemokines (MCP-1, M-CSF, MIP-2) in WAT. Some of these effects were more pronounced at a low dose of RC than at a large dose, and some of them were also canceled or changed in direction in animals treated with HFCD. In the amygdala, RC consumption increased the content of both pro- and antiinflammatory cytokines; the most significant was the increase in IL-7 levels in animals fed SD, and RANTES in animals fed HFCD. In the hippocampus of rats, the RC intake had an insignificant effect on the levels of cytokines and chemokines. Akt-1 kinase and the substrate of the insulin receptor IRS-1 were the main targets of RC action in the regions of the brain. Conclusion. The complex dietary supplement RC exerted a hypoleptinemic effect, revealed certain anti-inflammatory effects and modulated a number of the brain factors influencing behavioral responses in obesity. However, the synergistic effect of resveratrol and L-carnitine in the composition of the supplement wasn't not observed, and the effectiveness of its action decreased in conditions of a hypercaloric diet consumption.

[左旋肉碱和白藜芦醇复合物对正常大鼠和肥胖大鼠细胞因子和调节蛋白的影响]。
脂肪组织、外周器官和大脑某些区域的慢性炎症是肥胖症的致病因素之一。在专业产品和膳食补充剂的成分中使用具有抗炎和降血脂活性的生物活性化合物,被认为是肥胖症及相关疾病的饮食疗法的一种方法。本研究的目的是研究含有白藜芦醇和左旋肉碱的复合补充剂(RC 补充剂)对以平衡或高热量饮食喂养的大鼠的炎症免疫学参数(细胞因子和调节蛋白的概况)的影响。材料和方法雄性 Wistar 大鼠连续 63 天接受标准平衡膳食(SD)或总脂肪和果糖过量的高碳水化合物-高脂肪膳食(HFCD),以及低剂量(25 毫克/千克体重的 Res 和 300 毫克/千克体重的 L-Car)或高剂量(分别为 50 毫克/千克体重和 600 毫克/千克体重)的 RC 补充剂。通过多重免疫分析法研究了血清(BS)、白脂肪组织(WAT)和脾脏裂解物、大脑杏仁核和海马中瘦素、胃泌素、细胞因子和趋化因子的含量,以及杏仁核和海马中调节蛋白 Akt、IRS-1、GCK-3a/b、p70/S6、BAD、m-TOR、PTEN 和 S6 核糖体蛋白的含量。研究结果在摄入 RC 作为 SD 一部分的大鼠中,瘦素水平及其与胃泌素的比率、BS 和 WAT 中的促炎细胞因子(TNF-α、IL-1α、IL-12p40 和 IL-12p70、IFN-γ)水平、WAT 中的趋化因子(MCP-1、M-CSF、MIP-2)水平均有所下降。其中一些效应在低剂量 RC 时比大剂量时更明显,而且其中一些效应在使用 HFCD 的动物身上也被取消或改变了方向。在杏仁核中,摄入 RC 会增加促炎和抗炎细胞因子的含量;最显著的是摄入 SD 的动物 IL-7 含量增加,而摄入 HFCD 的动物 RANTES 含量增加。在大鼠的海马中,RC 摄入量对细胞因子和趋化因子的水平影响不大。Akt-1 激酶和胰岛素受体 IRS-1 底物是 RC 作用于大脑区域的主要目标。结论是复合膳食补充剂 RC 具有降胰岛素作用,显示出一定的抗炎效果,并能调节影响肥胖症行为反应的一些脑部因素。然而,并没有观察到白藜芦醇和左旋肉碱在补充剂成分中的协同作用,而且在摄入高热量饮食的情况下,其作用的有效性有所下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Voprosy pitaniia
Voprosy pitaniia Medicine-Medicine (all)
CiteScore
2.00
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0.00%
发文量
46
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