Scrub typhus in Indonesia: A cross-sectional analysis of archived fever studies samples.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-01 DOI:10.1093/trstmh/trad094

Kartika Saraswati, Ampai Tanganuchitcharnchai, Sirada Ongchaikupt, Mavuto Mukaka, Nicholas P J Day, J Kevin Baird, Ungke Antonjaya, Khin S A Myint, Yora P Dewi, Frilasita A Yudhaputri, Sotianingsih Haryanto, N P Diah Witari, Stuart D Blacksell

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引用次数: 0

Abstract

Background: Scrub typhus is an understudied vector-borne bacterial infection.

Methods: We tested archived fever samples for scrub typhus seropositivity to begin charting its geographic distribution in Indonesia. We analysed 1033 serum samples from three sites. IgM and IgG enzyme-linked immunosorbent assay (ELISA) against Orientia tsutsugamushi was performed using Karp, Kato, Gilliam, TA 716 antigens. To determine the cutoff in the absence of a presumed unexposed population and gold standard tests, we identified the visual inflection point, performed change point analysis, and used finite mixture models.

Results: The optical density cutoff values used for IgM and IgG were 0.49 and 0.13, respectively. Across all sites, IgM seropositivity was 4.6% (95% CI: 3.4 to 6.0%) while IgG seropositivity was 4.4% (95% CI: 3.3 to 5.8%). The overall seropositivity across sites was 8.8% (95% CI: 8.1 to 11.7%). The overall seropositivity for Jambi, Denpasar, Tabanan were 9.7% (95% CI: 7.0 to 13.3%), 8.0% (95% CI: 5.7 to 11.0%), 9.0% (95% CI: 6.1 to 13.0%), respectively.

Conclusions: We conclude that O. tsutsugamushi exposure in humans occurred at all sites analysed and could be the cause of illness in some cases. Though it was not the main cause of acute fever in these locations, it is still important to consider scrub typhus in cases not responding to beta-lactam antibiotics. Future seroprevalence surveys and testing for scrub typhus in acute febrile illness studies will be essential to understand its distribution and burden in Indonesia.

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印度尼西亚的恙虫病：对热病研究档案样本的横断面分析。

背景：恙虫病是一种未得到充分研究的病媒细菌感染：恙虫病是一种研究不足的病媒传播细菌感染：我们对存档的发热样本进行了恙虫病血清阳性检测，以开始绘制恙虫病在印度尼西亚的地理分布图。我们分析了来自三个地点的 1033 份血清样本。使用 Karp、Kato、Gilliam、TA 716 抗原进行了针对恙虫病的 IgM 和 IgG 酶联免疫吸附试验（ELISA）。为了在没有假定未暴露人群和金标准检测的情况下确定临界值，我们确定了视觉拐点，进行了变化点分析，并使用了有限混合物模型：结果：IgM 和 IgG 的光密度临界值分别为 0.49 和 0.13。在所有部位，IgM 血清阳性率为 4.6%（95% CI：3.4 至 6.0%），IgG 血清阳性率为 4.4%（95% CI：3.3 至 5.8%）。各地区的总体血清阳性率为 8.8%（95% CI：8.1% 至 11.7%）。占碑、登巴萨、塔巴南的总体血清阳性率分别为 9.7% (95% CI: 7.0 to 13.3%)、8.0% (95% CI: 5.7 to 11.0%)、9.0% (95% CI: 6.1 to 13.0%)：我们得出的结论是，人类接触恙虫病可能发生在所有分析地点，并可能是某些病例的致病原因。虽然恙虫病不是这些地区急性发热的主要病因，但在对β-内酰胺类抗生素无效的病例中考虑恙虫病仍然很重要。未来的血清流行率调查和急性发热疾病研究中的恙虫病检测对于了解恙虫病在印度尼西亚的分布和负担至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.