Effect of Polyphenols on the Ultrastructure of the Dentin Pellicle and Subsequent Erosion.

IF 2.9 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Caries Research Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI:10.1159/000536199

Anton Schestakow, Wadim Rasputnis, Matthias Hannig

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Abstract

Introduction: Erosive tooth wear is a highly prevalent dental condition that is modified by the ever-present salivary pellicle. The aim of the present in situ study was to investigate the effect of polyphenols on the ultrastructure of the pellicle formed on dentin in situ and a subsequent erosive challenge.

Methods: The pellicle was formed on bovine dentin specimens for 3 min or 2 h in 3 subjects. After subjects rinsed with sterile water (negative control), 1% tannic acid, 1% hop extract, or tin/fluoride solution containing 800 ppm tin and 500 ppm fluoride (positive control), specimens were removed from the oral cavity. The erosive challenge was performed on half of the specimens with 1% citric acid, and all specimens were analyzed by transmission electron microscopy. Incorporation of tannic acid in the pellicle was investigated by fluorescence spectroscopy.

Results: Compared to the negative control, ultrastructural analyses reveal a thicker and electron-denser pellicle after application of polyphenols, in which, according to spectroscopy, tannic acid is also incorporated. Application of citric acid resulted in demineralization of dentin, but to a lesser degree when the pellicle was pretreated with a tin/fluoride solution. The pellicle was more acid-resistant than the negative control when modified with polyphenols or tin/fluoride solution.

Conclusion: Polyphenols can have a substantial impact on the ultrastructure and acid resistance of the dentin pellicle, while the tin/fluoride solution showed explicit protection against erosive demineralization.

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多酚对牙本质薄层超微结构和随后侵蚀的影响。

介绍：腐蚀性牙齿磨损是一种非常普遍的牙科疾病，它是由持续存在的唾液胶粒改变的。本原位研究的目的是调查多酚对牙本质上原位形成的胶粒超微结构以及随后的侵蚀性挑战的影响：方法：在 3 名受试者的牛牙本质样本上形成 3 分钟或 2 小时的胶粒。受试者用无菌水（阴性对照组）、1%单宁酸、1%酒花提取物或含800 ppm锡和500 ppm氟的锡/氟溶液（阳性对照组）冲洗后，将标本从口腔中取出。用 1%柠檬酸对半数标本进行侵蚀性挑战，并用透射电子显微镜对所有标本进行分析。荧光光谱法研究了单宁酸在胶粒中的结合情况：结果：与阴性对照组相比，超微结构分析表明，施用多酚后的胶粒更厚，电子密度更高，根据光谱分析，单宁酸也融入了胶粒中。使用柠檬酸会导致牙本质脱矿，但使用锡/氟化物溶液预处理的胶层脱矿程度较低。结论：多酚可以对牙本质小泡的超微结构和耐酸性产生重大影响，而锡/氟化物溶液则对侵蚀性脱矿具有明显的保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caries Research 医学-牙科与口腔外科

CiteScore

6.30

自引率

7.10%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.