Ocular bioanalysis of moxifloxacin and ketorolac tromethamine in rabbit lacrimal matrix using liquid chromatography-tandem mass spectrometry.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-02-01 Epub Date: 2024-01-10 DOI:10.4155/bio-2023-0233

Amol Chhatrapati Bisen, Rabi Sankar Bhatta

引用次数: 0

Abstract

Aim: The fixed-dose combination of moxifloxacin (MOXI) and ketorolac tromethamine (KTR) is widely used for the treatment of bacterial keratitis. Thus, a new LC-MS/MS method was developed to determine MOXI and KTR in lacrimal fluid. Methods: Bioanalysis was performed using a Shimadzu 8050 LC-MS/MS in electrospray ionization-positive mode and the method was validated per US FDA guidelines. Isocratic separation was performed with a Waters Symmetry C₁₈ column using methanol and 0.1% formic acid containing deionized water (85:15, v/v). Results & conclusion: An easy, quick and selective method was established and applied to assess the ocular pharmacokinetic profile of a commercially available formulation containing MOXI and KTR. Based on the pharmacokinetic data, this work describes pharmacokinetics-based dosage regimen calculations and their clinical significance.

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利用液相色谱-串联质谱法对兔泪液基质中的莫西沙星和酮咯酸氨丁三醇进行眼部生物分析。

目的：莫西沙星（MOXI）和酮咯酸氨丁三醇（KTR）的固定剂量复方制剂被广泛用于治疗细菌性角膜炎。因此，我们开发了一种新的 LC-MS/MS 方法来测定泪液中的 MOXI 和 KTR。方法：使用岛津 8050 LC-MS/MS，在电喷雾离子化正离子模式下进行生物分析，并根据美国 FDA 指南对该方法进行了验证。采用 Waters Symmetry C18 色谱柱，以甲醇和含去离子水的 0.1% 甲酸（85:15, v/v）进行等度分离。结果与结论：本研究建立了一种简便、快速、选择性强的方法，用于评估含有 MOXI 和 KTR 的市售制剂的眼药代动力学特征。根据药代动力学数据，本研究阐述了基于药代动力学的剂量方案计算及其临床意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.