LC-MS/MS method for simultaneous estimation of raloxifene, cladrin in rat plasma: application in pharmacokinetic studies.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-02-01 Epub Date: 2024-01-10 DOI:10.4155/bio-2023-0206

Divya Chauhan, Shailesh Dadge, Pavan K Yadav, Nazneen Sultana, Arun Agarwal, Sachin Vishwakarma, Shivam Rathaur, Shubhi Yadav, Manish K Chourasia, Jiaur R Gayen

引用次数: 0

Abstract

Aim: A newer LC-MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v) as mobile phase by using biological matrices in female Sprague-Dawley rats using-MS/MS. Results: The developed method was found to be linear over the concentration ranges of 1-600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed C_max = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at T_max(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively. Conclusion: Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL for osteoporosis treatment.

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用 LC-MS/MS 方法同时估算大鼠血浆中的雷洛昔芬和克拉霉素：在药代动力学研究中的应用

目的：建立并验证一种新的 LC-MS/MS 方法，用于同时定量检测雷洛昔芬（RL）和氯羟菊酯（CL）。方法：采用RP-18 (4.6 × 50 mm, 5 μ) Xbridge Shield色谱柱，以乙腈和0.1%甲酸（FA）水溶液（70:30% v/v）为流动相，使用MS/MS对雌性Sprague-Dawley大鼠中的两种药物进行检测。结果表明所开发的方法在 1-600 纳克/毫升的浓度范围内线性良好，RL 和 CL 的定量下限分别为 1 纳克/毫升。RL+CL 的药代动力学结果显示，Cmax = 4.23 ± 0.61，26.97 ± 1.14 ng/ml，Tmax(h) 分别为 5.5 ± 1.00 和 3.5 ± 1.00。结论药代动力学研究结果将有助于今后将 RL 和 CL 联合用于骨质疏松症的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.