Lab on a chip for detecting Clara cell protein 16 (CC16) for potential screening of the workers exposed to respirable silica aerosol.

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Microfluidics and Nanofluidics Pub Date : 2023-11-01
Chong Ahn, Taekhee Lee, Jae Hoon Shin, Jong Seong Lee, V Thiyagarajan Upaassana, Sthitodhi Ghosh, Bon Ki Ku
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引用次数: 0

Abstract

Early detection of pulmonary responses to silica aerosol exposure, such as lung inflammation as well as early identification of silicosis initiation, is of great importance in disease prevention of workers. In this study, to early screen the health condition of the workers who are exposed to respirable silica dusts, an immunoassay lab on a chip (LOC) was designed, developed and fully characterized for analyzing Clara cell protein 16 (CC16) in serum which has been considered as one of the potential biomarkers of lung inflammation or lung damage due to the respirable silica dusts. Sandwich immunoassay of CC16 was performed on the LOC developed with a custom-designed portable analyzer using artificial serums spiked with CC16 protein first and then human serums obtained from the coal mine workers exposed to the respirable silica-containing dusts. The dynamic range of CC16 assay performed on the LOC was in a range of 0.625-20 ng/mL, and the achieved limit of detection (LOD) was around 0.35 ng/mL. The assay results of CC16 achieved from both the developed LOC and the conventional 96 well plate showed a reasonable corelation. The correlation between the conventional reader and the developed portable analyzer was found to be reasonable, resulting in R2 ~ 0.93. This study shows that the LOC developed for the early detection of CC16 can be potentially applied for the development of a field-deployable point-of-care testing (POCT) for the early monitoring of the field workers who are exposed to silica aerosol.

用于检测克拉拉细胞蛋白 16(CC16)的芯片实验室,可用于筛查暴露于可吸入二氧化硅气溶胶的工人。
早期检测接触二氧化硅气溶胶的肺部反应(如肺部炎症)以及早期识别矽肺病的诱发因素,对工人的疾病预防具有重要意义。为了早期筛查暴露于可吸入二氧化硅粉尘的工人的健康状况,本研究设计、开发了一种芯片上免疫测定实验室(LOC),并对其进行了全面鉴定,用于分析血清中的克拉细胞蛋白 16(CC16),该蛋白被认为是可吸入二氧化硅粉尘导致肺部炎症或肺损伤的潜在生物标志物之一。首先使用添加了 CC16 蛋白的人工血清,然后使用从暴露于含可吸入二氧化硅粉尘的煤矿工人处获得的人体血清,在定制设计的便携式分析仪开发的 LOC 上进行了 CC16 的三明治免疫测定。在 LOC 上进行的 CC16 检测的动态范围为 0.625-20 纳克/毫升,检出限(LOD)约为 0.35 纳克/毫升。开发的 LOC 和传统 96 孔板的 CC16 检测结果显示出合理的相关性。传统阅读器与开发的便携式分析仪之间的相关性也很合理,R2 ~ 0.93。这项研究表明,所开发的用于早期检测 CC16 的 LOC 有可能被用于开发一种可现场部署的护理点检测(POCT),以对暴露于二氧化硅气溶胶的现场工作人员进行早期监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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