High glucose regulates the cells dysfunction of human trophoblast HTR8/SVneo cells by downregulating GABRP expression.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Jianping Wang, Lianyun Wang, Haifan Qiu
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Abstract

Background: In response to the high-glucose environment in patients with gestational diabetes mellitus (GDM), trophoblast cells undergo a series of pathological changes. Gamma-aminobutyric acid type A receptor subunit pi (GABRP) is involved in the development of pregnancy-related diseases and regulation of blood glucose.

Objectives: To explore the relationship between GABRP and hyperglycemia stimulation in GDM patients, and to provide preliminary experimental evidence for whether GABRP has the potential as a molecular target for the treatment of GDM.

Material and methods: Within 30 min after birth, placental samples were taken from 20 GDM patients and 20 pregnant women without GDM. Human chorionic trophoblast HTR-8/SVneo cells were utilized for in vitro experimental investigation. We explored changes in GABRP expression in placental samples and HTR-8/Svneo cells using western blot and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Cells in the high-glucose treatment group were exposed to medium containing 25 mM glucose. To explore the relationship between GABRP and high-glucose stimulation, GABRP was overexpressed in HTR-8/SVneo cells. We monitored the cell viability, invasion and migration abilities using Cell Counting Kit-8 (CCK-8), transwell and scratch assays, respectively.

Results: We found that GABRP expression was significantly reduced in placental samples from GDM patients. Furthermore, high-glucose treatment decreased the expression level of GABRP in HTR-8/SVneo cells. High-glucose stimulation reduced the cell viability, invasion and migration abilities. GABRP overexpression reversed the biological dysfunction of the cells induced by high-glucose stimulation.

Conclusions: Hyperglycemia in GDM patients downregulates the expression of GABRP, and overexpression of GABRP promotes the viability, migration and invasive ability of HTR8-/SVneo cells.

高糖通过下调 GABRP 的表达调节人滋养层细胞 HTR8/SVneo 的细胞功能障碍。
背景:为应对妊娠糖尿病(GDM)患者的高血糖环境,滋养层细胞发生了一系列病理变化。γ-氨基丁酸 A 型受体亚基 pi(GABRP)参与妊娠相关疾病的发生和血糖的调节:探讨GABRP与GDM患者高血糖刺激的关系,并为GABRP是否有可能成为治疗GDM的分子靶点提供初步的实验证据:在胎儿出生后 30 分钟内,从 20 名 GDM 患者和 20 名未患 GDM 的孕妇身上采集胎盘样本。利用人体绒毛滋养层细胞 HTR-8/SVneo 进行体外实验研究。我们使用 Western 印迹和定量反转录聚合酶链反应(RT-qPCR)技术探讨了胎盘样本和 HTR-8/Svneo 细胞中 GABRP 表达的变化。高葡萄糖处理组的细胞暴露于含 25 mM 葡萄糖的培养基中。为了探索 GABRP 与高葡萄糖刺激之间的关系,我们在 HTR-8/SVneo 细胞中过表达了 GABRP。我们分别使用细胞计数试剂盒-8(CCK-8)、透孔法和划痕法监测了细胞的活力、侵袭和迁移能力:结果:我们发现 GDM 患者的胎盘样本中 GABRP 的表达明显减少。此外,高葡萄糖处理降低了 GABRP 在 HTR-8/SVneo 细胞中的表达水平。高葡萄糖刺激降低了细胞的活力、侵袭和迁移能力。GABRP的过表达可逆转高糖刺激引起的细胞生物功能障碍:结论:GDM 患者的高血糖会下调 GABRP 的表达,而 GABRP 的过表达会提高 HTR8-/SVneo 细胞的活力、迁移和侵袭能力。
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来源期刊
Advances in Clinical and Experimental Medicine
Advances in Clinical and Experimental Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
3.70
自引率
4.80%
发文量
153
审稿时长
6-12 weeks
期刊介绍: Advances in Clinical and Experimental Medicine has been published by the Wroclaw Medical University since 1992. Establishing the medical journal was the idea of Prof. Bogumił Halawa, Chair of the Department of Cardiology, and was fully supported by the Rector of Wroclaw Medical University, Prof. Zbigniew Knapik. Prof. Halawa was also the first editor-in-chief, between 1992-1997. The journal, then entitled "Postępy Medycyny Klinicznej i Doświadczalnej", appeared quarterly. Prof. Leszek Paradowski was editor-in-chief from 1997-1999. In 1998 he initiated alterations in the profile and cover design of the journal which were accepted by the Editorial Board. The title was changed to Advances in Clinical and Experimental Medicine. Articles in English were welcomed. A number of outstanding representatives of medical science from Poland and abroad were invited to participate in the newly established International Editorial Staff. Prof. Antonina Harłozińska-Szmyrka was editor-in-chief in years 2000-2005, in years 2006-2007 once again prof. Leszek Paradowski and prof. Maria Podolak-Dawidziak was editor-in-chief in years 2008-2016. Since 2017 the editor-in chief is prof. Maciej Bagłaj. Since July 2005, original papers have been published only in English. Case reports are no longer accepted. The manuscripts are reviewed by two independent reviewers and a statistical reviewer, and English texts are proofread by a native speaker. The journal has been indexed in several databases: Scopus, Ulrich’sTM International Periodicals Directory, Index Copernicus and since 2007 in Thomson Reuters databases: Science Citation Index Expanded i Journal Citation Reports/Science Edition. In 2010 the journal obtained Impact Factor which is now 1.179 pts. Articles published in the journal are worth 15 points among Polish journals according to the Polish Committee for Scientific Research and 169.43 points according to the Index Copernicus. Since November 7, 2012, Advances in Clinical and Experimental Medicine has been indexed and included in National Library of Medicine’s MEDLINE database. English abstracts printed in the journal are included and searchable using PubMed http://www.ncbi.nlm.nih.gov/pubmed.
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