Capped or uncapped? Techniques to assess the quality of mRNA molecules

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Systems Biology Pub Date : 2024-01-04 DOI:10.1016/j.coisb.2023.100503

Ying Tu, Akashaditya Das, Chileab Redwood-Sawyerr, Karen M. Polizzi

引用次数: 0

Abstract

The successful use of mRNA vaccines during the Covid-19 pandemic has created a boom in mRNA therapeutic research and development. The efficacy of mRNA vaccines and therapies relies on the quality of the synthesized molecules – a key feature of which is the 5′-end cap modification. The development of analytical methods for assessing mRNA quality needs to be prioritized to enable manufacturing development, process control, and rapid assessment of batch quality before release. In this review, we provide an overview of the latest techniques in the analysis of mRNA 5′ capping. We also discuss future possibilities and challenges in quality control of mRNA products at scale.

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有封顶还是无封顶？评估 mRNA 分子质量的技术

在 Covid-19 大流行期间，mRNA 疫苗的成功使用掀起了 mRNA 疗法研究与开发的热潮。mRNA 疫苗和疗法的疗效取决于合成分子的质量，其中一个关键特征是 5′端帽修饰。需要优先发展评估 mRNA 质量的分析方法，以便进行生产开发、过程控制和发布前批次质量的快速评估。在本综述中，我们概述了分析 mRNA 5′ 端封帽的最新技术。我们还讨论了大规模 mRNA 产品质量控制的未来可能性和挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Systems Biology Mathematics-Applied Mathematics

CiteScore

7.10

自引率

2.70%

发文量

期刊介绍： Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution