Impacts of H2O2, SARM1 inhibition, and high NAm concentrations on Huntington's disease laser-induced degeneration

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2024-01-07 DOI:10.1002/jbio.202300370

Sophia Barber, Veronica Gomez-Godinez, Joy Young, Abigail Wei, Sarah Chen, Anna Snissarenko, Sze Sze Chan, Chengbiao Wu, Linda Shi

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Abstract

Axonal degeneration is a key component of neurodegenerative diseases such as Huntington's disease (HD), Alzheimer's disease, and amyotrophic lateral sclerosis. Nicotinamide, an NAD+ precursor, has long since been implicated in axonal protection and reduction of degeneration. However, studies on nicotinamide (NAm) supplementation in humans indicate that NAm has no protective effect. Sterile alpha and toll/interleukin receptor motif-containing protein 1 (SARM1) regulates several cell responses to axonal damage and has been implicated in promoting neuronal degeneration. SARM1 inhibition seems to result in protection from neuronal degeneration while hydrogen peroxide has been implicated in oxidative stress and axonal degeneration. The effects of laser-induced axonal damage in wild-type and HD dorsal root ganglion cells treated with NAm, hydrogen peroxide (H₂O₂), and SARM1 inhibitor DSRM-3716 were investigated and the cell body width, axon width, axonal strength, and axon shrinkage post laser-induced injury were measured.

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H2 O2、SARM1 抑制剂和高浓度 NAm 对亨廷顿氏病激光诱导变性的影响。

轴突变性是亨廷顿氏病（HD）、阿尔茨海默病和肌萎缩侧索硬化症等神经退行性疾病的关键组成部分。烟酰胺是一种 NAD+ 前体，很早就被认为与保护轴突和减少退化有关。然而，对人体补充烟酰胺（NAm）的研究表明，NAm 没有保护作用。不育α和含通行费/白细胞介素受体基序蛋白1（SARM1）调节多种细胞对轴突损伤的反应，并与促进神经元退化有关。抑制 SARM1 似乎能防止神经元变性，而过氧化氢则与氧化应激和轴突变性有关。研究人员用NAm、过氧化氢（H2 O2）和SARM1抑制剂DSRM-3716处理了野生型和HD背根神经节细胞，并测量了激光诱导轴突损伤后细胞体宽度、轴突宽度、轴突强度和轴突收缩的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.