Nocardioides jiangxiensis sp. nov., a novel actinobacterium isolated from lakeside soil, exhibiting the biosynthesis potential of mycofactocin

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-08 DOI:10.1007/s10482-023-01910-4

Yingying Wan, Tianli Sun, Guangwen Huang, Bance Tu, Mingzhu Huang, Xuelan Chen, Bin Liu, Jian He

{"title":"Nocardioides jiangxiensis sp. nov., a novel actinobacterium isolated from lakeside soil, exhibiting the biosynthesis potential of mycofactocin","authors":"Yingying Wan, Tianli Sun, Guangwen Huang, Bance Tu, Mingzhu Huang, Xuelan Chen, Bin Liu, Jian He","doi":"10.1007/s10482-023-01910-4","DOIUrl":null,"url":null,"abstract":"<div>A Gram-stain-positive, rod-shaped, non-spore-forming and non-motile bacterium, designated WY-20T, was isolated from a lakeside soil sample collected in Jiangxi Province, PR China. Growth was observed at 20–42 °C (optimum 30 °C), pH 5.0–8.0 (optimum pH 7.0) and salinity of 0–3.0% (w/v; optimum 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain WY-20T belongs to the genus Nocardioides and showed the highest sequence similarity (98.1%) to N. phosphati WYH11-7T, followed by N. cavernaquae K1W22B-1T (97.8%), N. marmoriterrae JOS5-1T (97.2%) and N. jensenii NBRC 14755T (97.1%). The average nucleotide identity and digital DNA–DNA hybridization values between strains WY-20T and N. phosphati WYH11-7T were 83.5% and 26.2%, respectively. The predominant fatty acids (≥ 10% of the total fatty acids) were C18:1ω9c, C17:0, C16:0, summed feature 8 (C18:1ω7c and/or C18: 1ω6c) and C17:1ω9c. The major menaquinone was MK-8 (H4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Based on phenotypic, genotypic and phylogenetic pieces of evidence, strain WY-20T represents a novel species in the genus Nocardioides, for which the name Nocardioides jiangxiensis sp. nov. is proposed. The type strain is WY-20T (= GDMCC 4.317T = KACC 23379T).</div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-023-01910-4","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

A Gram-stain-positive, rod-shaped, non-spore-forming and non-motile bacterium, designated WY-20^T, was isolated from a lakeside soil sample collected in Jiangxi Province, PR China. Growth was observed at 20–42 °C (optimum 30 °C), pH 5.0–8.0 (optimum pH 7.0) and salinity of 0–3.0% (w/v; optimum 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain WY-20^T belongs to the genus Nocardioides and showed the highest sequence similarity (98.1%) to N. phosphati WYH11-7^T, followed by N. cavernaquae K1W22B-1^T (97.8%), N. marmoriterrae JOS5-1^T (97.2%) and N. jensenii NBRC 14755^T (97.1%). The average nucleotide identity and digital DNA–DNA hybridization values between strains WY-20^T and N. phosphati WYH11-7^T were 83.5% and 26.2%, respectively. The predominant fatty acids (≥ 10% of the total fatty acids) were C_18:1ω9c, C_17:0, C_16:0, summed feature 8 (C_18:1ω7c and/or C_18: 1ω6c) and C_17:1ω9c. The major menaquinone was MK-8 (H4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Based on phenotypic, genotypic and phylogenetic pieces of evidence, strain WY-20^T represents a novel species in the genus Nocardioides, for which the name Nocardioides jiangxiensis sp. nov. is proposed. The type strain is WY-20^T (= GDMCC 4.317^T = KACC 23379^T).

Abstract Image

查看原文本刊更多论文

从湖边土壤中分离出的新型放线菌 Nocardioides jiangxiensis sp.

从中国江西省采集的湖边土壤样本中分离出一种革兰氏染色阳性、杆状、无芽孢、不运动的细菌，命名为 WY-20T。该细菌在 20-42 °C（最适温度为 30 °C）、pH 值为 5.0-8.0 （最适 pH 值为 7.0）、盐度为 0-3.0% （w/v；最适盐度为 0.5%）的条件下生长。基于 16S rRNA 基因序列的系统进化分析表明，菌株 WY-20T 属于 Nocardioides 属，与 N. phosphati WYH11-7T 的序列相似度最高（98.1%），其次是 N. cavernaquae K1W22B-1T (97.8%)、N. marmoriterrae JOS5-1T (97.2%) 和 N. jensenii NBRC 14755T (97.1%)。WY-20T 和 N. phosphati WYH11-7T 菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 83.5% 和 26.2%。主要脂肪酸（≥总脂肪酸的 10%）为 C18:1ω9c、C17:0、C16:0、总特征 8（C18:1ω7c 和/或 C18: 1ω6c）和 C17:1ω9c。主要的甲萘醌是 MK-8（H4）。主要的极性脂质是二磷脂酰甘油、磷脂酰甘油和两种不明磷脂。此外，细胞壁肽聚糖中的中二氨基亚氨嘧啶酸（meso-diaminopimelic acid）具有诊断意义。根据表型、基因型和系统发育的证据，WY-20T 株系代表了 Nocardioides 属中的一个新物种，并将其命名为 Nocardioides jiangxiensis sp.模式菌株为 WY-20T（= GDMCC 4.317T = KACC 23379T）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.