Dual labelled probe assays for differentiation of Botryosphaeria dothidea, Neofusicoccum mediterraneum and Neofusicoccum parvum, based on polymorphisms in the MAT1-2-1 gene

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2023-07-23 DOI:10.36253/phyto-14375

S. Palavouzis, Alexandra Triantafyllopoulou, Christos Tsoukas, A. Tzima, E. Paplomatas

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Abstract

Botryosphaeriaceous fungi are widespread, and cause serious diseases in many economically important crops. Botryosphaeria dothidea, Neofusicoccum mediterraneum and N. parvum are the most important members of this family in the Mediterranean region. These fungi are frequently isolated from the same host, which together with their extensive and increasing host range necessitates development of rapid and reliable diagnostic tools. Species boundaries within the Botryosphaeriaceae have been defined based on phylogenetic analyses of multiple gene sequences, including those of mating type genes. The MAT1-2-1 gene displayed high sequence variability between Botryosphaeriaceous species, so was selected as the target for development of a definitive diagnostic tool. This paper outlines a new and robust molecular tool, composed of three TaqMan assays based on polymorphisms located in the MAT1-2-1 gene of B. dothidea, N. mediterraneum and N. parvum. Each assay differentiated the target species from other Botryosphaeriaceae, and from non-target fungi.

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基于 MAT1-2-1 基因多态性的双标记探针测定法，用于区分 Botryosphaeria dothidea、Neofusicoccum mediterraneum 和 Neofusicoccum parvum

Botryosphaeriaceous 真菌分布广泛，对许多具有重要经济价值的作物造成严重病害。Botryosphaeria dothidea、Neofusicoccum mediterraneum 和 N. parvum 是该家族在地中海地区最重要的成员。这些真菌经常从同一寄主中分离出来，再加上它们的寄主范围越来越广，因此有必要开发快速可靠的诊断工具。根据多基因序列（包括交配型基因序列）的系统发育分析，确定了 Botryosphaeriaceae 的物种界限。MAT1-2-1 基因在 Botryosphaeriaceous 物种之间显示出较高的序列变异性，因此被选为开发明确诊断工具的目标。本文概述了一种新的、强大的分子工具，它由三种基于 B. dothidea、N. mediterraneum 和 N. parvum MAT1-2-1 基因多态性的 TaqMan 检测法组成。每种检测方法都能将目标物种与其他 Botryosphaeriaceae 以及非目标真菌区分开来。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.