Fusarium oxysporum f. sp. lycopersici biomass variations under disease control regimes using Trichoderma and compost

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2023-07-23 DOI:10.36253/phyto-14016

A. Saleh, Arya Widyawan, A. Sharafaddin, A. Almasrahi, Y. Hamad

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引用次数: 0

Abstract

A comprehensive understanding of population dynamics of pathogens and bioagents in plant rhizospheres is important for improving organic farming. Fusarium oxysporum f. sp. lycopersici (FOL30) causes Fusarium wilt of tomato. In this study, we compared biomass variations of FOL30 under different disease control regimes, using Trichoderma asperellum TA23 strain, compost, or their combination. Biomass variations of FOL30 and TA23 were observed for 13 weeks using quantitative real-time PCR. Separate applications of TA23, compost, and their combination all reduced FOL biomass when compared to experimental controls. Regression analyses of the qPCR data showed that FOL populations fitted curvilinear polynomial order 3 regression models (R2 = 0.87 to 0.95). Areas under the population dynamic curves (AUPDCs; log10 ng DNA week-1 g-1 soil) were: 43.8 from FOL30 alone, 36.6 from FOL30 plus TA23, 25.4 from FOL30 plus compost, and 25.5 from FOL30 plus TA23 plus compost. These results indicate that the individual applications of TA23 or compost, or their combination, decreased the FOL biomass. The negative correlation between TA23 and FOL30 populations showed that the compost and biocontrol agent reduced FOL pathogen populations. This study demonstrates that compost fortified with T. asperellum TA23 decreased FOL populations and reduced disease, and that their use is a promising strategy for managing Fusarium wilt of tomato in organic farming.

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使用毛霉菌和堆肥控制病害情况下的 Fusarium oxysporum f. sp. lycopersici 生物量变化

全面了解植物根瘤中病原体和生物制剂的种群动态对改善有机耕作非常重要。Fusarium oxysporum f. sp. lycopersici（FOL30）会导致番茄镰刀菌枯萎病。在这项研究中，我们比较了 FOL30 在不同病害控制制度下的生物量变化，包括使用赤霉菌 TA23 菌株、堆肥或它们的组合。使用实时定量 PCR 技术对 FOL30 和 TA23 的生物量变化进行了 13 周的观察。与实验对照组相比，分别施用 TA23、堆肥和它们的组合都降低了 FOL 的生物量。对 qPCR 数据的回归分析表明，FOL 种群符合曲线多项式 3 阶回归模型（R2 = 0.87 至 0.95）。种群动态曲线下的面积（AUPDCs；log10 ng DNA week-1 g-1 soil）分别为单独施用 FOL30 为 43.8，FOL30 加 TA23 为 36.6，FOL30 加堆肥为 25.4，FOL30 加 TA23 加堆肥为 25.5。这些结果表明，单独施用 TA23 或堆肥，或两者结合施用，都会减少 FOL 的生物量。TA23 和 FOL30 的数量呈负相关，这表明堆肥和生物控制剂减少了 FOL 的病原体数量。这项研究表明，堆肥中添加灰孢镰刀菌 TA23 可减少 FOL 的数量并减轻病害，是有机农业中管理番茄镰刀菌枯萎病的一种有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.