Molecular authentication of Thevetia peruviana (pers.) roxb plant by random amplified polymorphic deoxyribonucleic acid – Barcoding technique

Abstract

BACKGROUND: DNA fingerprinting is a Special technique used to determine the probable identity of a person based on the nucleotide sequences of certain regions of Plant DNA that are unique to individuals. AIMS AND OBJECTIVES: In this study, the random amplified polymorphic deoxyribonucleic acid (RAPD) technique was employed for the determination of the components in Ayurvedic herbal medicine. The RAPD approach is appealing to a large number of scientists since it may be implemented in a variety of settings with little effort. Most importantly, RAPD analysis does not require any cloning, sequencing, or other molecular characterization of the genome to extract a large number of genetic markers from a small amount of deoxyribonucleic acid (DNA). MATERIALS AND METHODS: Fresh leaves of the Thevetia peruviana (Pers.) Roxb plants were gathered in the month of June in the surroundings of Solapur. RESULTS: We used OPAB11, OPT12, OPQ15, and OPC104 decamer primers to conduct an RAPD study that measures genetic variation at random. Pure and RNA free high molecular weight DNA was extracted from the leaves. CONCLUSION: The DNA was amplified with repeat primers and 4 polymerase chain reaction primers, and then analyzed on agarose gels. Other wild T. peruviana species might be easier to track down with this method.