INHIBITION OF ALPHA-GLUCOSIDASE BY POLYSACCHARIDES FROM WATER LETTUCE (Pistia stratiotes) LEAF EXTRACTS WITH DIFFERENT EXTRACTION METHODS

IF 0.6 Q4 FOOD SCIENCE & TECHNOLOGY

Journal of microbiology, biotechnology and food sciences Pub Date : 2023-10-04 DOI:10.55251/jmbfs.9805

S. Sudirman, Aatikah Dewi Ghaisani, Indah Widiastuti, Herpandi Herpandi, Yohana Novelina Sirat, Miftahul Janna

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引用次数: 0

Abstract

Diabetes mellitus (DM) is a metabolic disorder that has a significant impact on the quality of life and health. This condition is indicated by high blood glucose or hyperglycemia. Alpha-glucosidase is an important enzyme during carbohydrate digestion to obtain monosaccharides such as glucose. This study aimed to investigate the alpha-glucoside inhibitory activity of polysaccharides from water lettuce (Pistia stratiotes) leaf with different extraction methods. The leaf was extracted by hot water extraction (HWE) and ultrasound-assisted extraction (UAE). The total sugar, crude fiber, and alpha-glucosidase inhibitory activity were analysis by in vitro, then an independent-sample t-test was used to analyze the statistically different. The UAE method showed a high yield of crude extract than the HWE method. However, the total sugar of HWE is higher than UAE, whereas no different of crude fiber level. The extract from the HWE method more effectively inhibits the alpha-glucosidase activity when compared to UAE. Therefore, hot water extraction is the suitable method to extract polysaccharides from a water lettuce (Pistia stratiotes) leaf. This condition also indicated polysaccharides extract potential to develop as an antidiabetes agent.

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用不同的提取方法从水葫芦（Pistia stratiotes）叶提取物中提取多聚乙烯羧酸对α-葡糖苷酶的抑制作用

糖尿病（DM）是一种对生活质量和健康有重大影响的代谢性疾病。这种病症表现为高血糖或高血糖。 α-葡萄糖苷酶是消化碳水化合物以获得葡萄糖等单糖的一种重要酶。本研究旨在探讨不同提取方法对水生莴苣（Pistia stratiotes）叶片中多糖的α-葡萄糖苷酶抑制活性。莴苣叶采用热水提取法（HWE）和超声辅助提取法（UAE）提取。对总糖、粗纤维和α-葡萄糖苷酶抑制活性进行了体外分析，然后采用独立样本 t 检验对统计学差异进行分析。结果表明，UAE 法比 HWE 法的粗提物产量高。不过，HWE 法的总糖含量高于 UAE 法，而粗纤维含量则没有差异。与 UAE 法相比，HWE 法提取物能更有效地抑制α-葡萄糖苷酶的活性。因此，热水提取法是提取莴苣叶多糖的合适方法。这一条件也表明多糖提取物具有作为抗糖尿病药物的潜力。

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来源期刊

Journal of microbiology, biotechnology and food sciences FOOD SCIENCE & TECHNOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

156

审稿时长

8 weeks

期刊介绍： The Journal of Microbiology, Biotechnology and Food Sciences is an Open Access, peer-reviewed online scientific journal published by the Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra). The major focus of the journal is regular publishing of original scientific articles, short communications and reviews about animal, plant and environmental microbiology (including bacteria, fungi, yeasts, algae, protozoa and viruses), microbial, animal and plant biotechnology and physiology, microbial, plant and animal genetics, molecular biology, agriculture and food chemistry and biochemistry, food control, evaluation and processing in food science and environmental sciences.