FORMULATION AND EVALUATION OF PHYTOSOMES LOADED WITH PITHECELLOBIUM BIJENINUM LEAF EXTRACT

Current Research in Pharmaceutical Sciences Pub Date : 2023-10-08 DOI:10.24092/crps.2023.130306

Arihant Jain, Richa Verma, Parulben D Mehta

{"title":"FORMULATION AND EVALUATION OF PHYTOSOMES LOADED WITH PITHECELLOBIUM BIJENINUM LEAF EXTRACT","authors":"Arihant Jain, Richa Verma, Parulben D Mehta","doi":"10.24092/crps.2023.130306","DOIUrl":null,"url":null,"abstract":"The objective of the present study was to prepare phytosomal formulation loaded with aqueous extract of Pithecellobium bijeninum leaves. The extraction of leaf powder was done by maceration technique using 90:10 ethanol-water mixture with an extraction yield of 6.12%. The extract was found to contain alkaloids, and flavonoids and the total phenolic content was found to be 23.2 ± 0.827 %w/w. Phytosomes of the extract were prepared by solvent evaporation method using lecithin as the lipid molecule. The particle size of the phytosomes was from 468 nm to 1827 nm in size with a polydispersity index varying between 0.178 - 0.464. The phytosomes were visible as rigid, almost spherical vesicles in SEM image. The surface of the phytosome vesicles was found to be regular and smooth. The point prediction suggested level 3 (0.3 g) of lipid concentration and level 2 (0.2 g) of extract concentration to present the lowest particle size. Sharp and distinct endothermic peaks in DSC revealed the formulation of stable phytosomes due to molecular interactions between the extract and lecithin. The phytosomes were found to possess good antioxidant action against DPPH radical in the in vitro scavenging assay. The IC50 value of the extract against DPPH was found to be 48.24 µg and that of the phytosomal formulation loaded with the extract (F4) was obtained to be 48.90 µg. KEYWORDS: Phytosome, extraction, lecithin, Pithecellobium, antioxidant","PeriodicalId":11053,"journal":{"name":"Current Research in Pharmaceutical Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Research in Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24092/crps.2023.130306","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The objective of the present study was to prepare phytosomal formulation loaded with aqueous extract of Pithecellobium bijeninum leaves. The extraction of leaf powder was done by maceration technique using 90:10 ethanol-water mixture with an extraction yield of 6.12%. The extract was found to contain alkaloids, and flavonoids and the total phenolic content was found to be 23.2 ± 0.827 %w/w. Phytosomes of the extract were prepared by solvent evaporation method using lecithin as the lipid molecule. The particle size of the phytosomes was from 468 nm to 1827 nm in size with a polydispersity index varying between 0.178 - 0.464. The phytosomes were visible as rigid, almost spherical vesicles in SEM image. The surface of the phytosome vesicles was found to be regular and smooth. The point prediction suggested level 3 (0.3 g) of lipid concentration and level 2 (0.2 g) of extract concentration to present the lowest particle size. Sharp and distinct endothermic peaks in DSC revealed the formulation of stable phytosomes due to molecular interactions between the extract and lecithin. The phytosomes were found to possess good antioxidant action against DPPH radical in the in vitro scavenging assay. The IC50 value of the extract against DPPH was found to be 48.24 µg and that of the phytosomal formulation loaded with the extract (F4) was obtained to be 48.90 µg. KEYWORDS: Phytosome, extraction, lecithin, Pithecellobium, antioxidant

查看原文本刊更多论文

装载坑叶栲叶的植物体的制备与评估

本研究的目的是制备含有苦参叶水提取物的植物体制剂。采用浸渍技术，使用 90:10 的乙醇-水混合物萃取叶粉，萃取率为 6.12%。提取物中含有生物碱和黄酮类化合物，总酚含量为 23.2 ± 0.827 %w/w。以卵磷脂为脂质分子，通过溶剂蒸发法制备了提取物的植物体。植物体的粒径在 468 nm 到 1827 nm 之间，多分散指数在 0.178 - 0.464 之间。在扫描电镜图像中，可以看到植物体呈硬质、近似球形的囊泡。植物囊泡的表面规则而光滑。点预测表明，脂质浓度为 3 级（0.3 克）和提取物浓度为 2 级（0.2 克）时，粒径最小。由于提取物和卵磷脂之间的分子相互作用，在 DSC 中出现了尖锐而明显的内热峰，这表明制备出了稳定的植物体。体外清除试验发现，植物体对 DPPH 自由基具有良好的抗氧化作用。提取物对 DPPH 的 IC50 值为 48.24 µg，载入提取物的植物体制剂（F4）的 IC50 值为 48.90 µg。关键词：植物体、萃取、卵磷脂、苦参碱、抗氧化剂

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Research in Pharmaceutical Sciences

自引率

0.00%

发文量