Neuroprotective Potential of Orientin with Antiepileptic Drugs against Pentylenetetrazole-induced Kindling Model and Evaluation of Behavioral Assessment in Mice

Abstract

The neuroprotective effect of bioflavonoids has been demonstrated in epileptic disorder. The objective of this study was to investigate the anticonvulsant and adjuvant effects of the bioflavonoid and explore behavioural responses of orientin (Ore) on kindled mice induced by pentylenetetrazole [PTZ]. Albino Swiss mice weighing 20-30 g were divided into nine groups [n=6]. Prior to the PTZ dose, alternatively, ore [10 mg/kg, i.p.] was given for 7 days, dissolved in 6% w/v carboxymethylcellulose [CMC] salt. On the 7th day, saline was solubilized with Lamotrigine [Lmt], Phenobarbital [Pb], and Gabapentin [Gbp] and administered as separate intraperitoneal [i.p.] injections 30 minutes prior to the PTZ dose. For the development of kindling seizures in mice, PTZ [30 mg/kg, i.p.] was delivered to all the mice for 12 days, alternatively until the animals appeared to develop full motor muscle jerking seizures. Mice who survived from complete motor seizures were selected for further experimentation. Data showed that anticonvulsive activity was exhibited by the control. Ore [10 mg/kg] with PB [40 mg/kg, i.p.] was administered on the 12th day and showed an increase in transfer delays [ITL and RTL]. Anti-seizure efficacy of drugs was investigated at the effective dose of ore at 10 mg/kg + PB 40mg/kg in group 7 and was found to have promising therapeutic outcomes and potency in therapeutic strategies and associated concerns.