The Relationship Between Morphometric Characteristics and The Weight of Tubuca forcipata (Subfamily: Gelasiminae) in The Mangrove Nature Reserve of Eastern Coast Resort Mendahara, Jambi Province

Tazkiah Auliaputri, Mahya Ihsan, Tia Wulandari
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Abstract

The objective of this study is to ascertain the pathogenic and antagonistic fungi that induce disease in avocado plants (Persea americana Mill), identify the antagonistic fungi with the greatest antagonism potential among a variety of antagonistic fungi, and elucidate the mechanism by which antagonistic fungi inhibit the growth of pathogenic fungi. From January to May 2023, this study was conducted in vitro at the Microbiology Laboratory, State University of Malang, employing a completely randomized design (CRD) comprising six replications and four interventions. The identification of mold was accomplished through macroscopically and microscopically descriptive analysis, followed by a comparison with the mold identification key book. The antagonism process was executed utilizing the dual culture method on the PDA medium, and the electron microscope (SEM) was employed to witness the antagonism mechanism. The research findings revealed that T. harzianum spp. and T. viride were identified as antagonist fungi, whereas C. aotearoa and C. gloeospoiroides were identified as pathogenic fungi. The antagonistic fungus T. viride exhibited the greatest degree of antagonism, impeding the growth of C. gloeospoiroides by 77.8%. Observed antagonism occurs when the hyphae of the antagonistic mold ensnare, entangle, and pierce those of the pathogenic mold, causing harm to the region surrounding the pathogenic mold's hyphae and subsequently impeding its growth
占碑省东部海岸度假村门达哈拉红树林自然保护区中的 Tubuca forcipata(Gelasiminae 亚科)形态特征与体重之间的关系
本研究的目的是确定诱发牛油果植物(Persea americana Mill)病害的病原真菌和拮抗真菌,从多种拮抗真菌中找出拮抗潜力最大的真菌,并阐明拮抗真菌抑制病原真菌生长的机制。这项研究于 2023 年 1 月至 5 月在马朗州立大学微生物实验室进行,采用完全随机设计(CRD),包括六次重复和四次干预。霉菌的鉴定是通过宏观和微观描述性分析完成的,然后与霉菌鉴定手册进行比较。在 PDA 培养基上采用双重培养法进行拮抗过程,并使用电子显微镜(SEM)观察拮抗机制。研究结果表明,T. harzianum spp.和 T. viride 被确定为拮抗真菌,而 C. aotearoa 和 C. gloeospoiroides 被确定为致病真菌。拮抗真菌 T. viride 表现出最大程度的拮抗作用,对 C. gloeospoiroides 的生长阻碍达 77.8%。当拮抗霉菌的菌丝缠住、缠绕并刺穿病原霉菌的菌丝时,就会产生拮抗作用,对病原霉菌菌丝周围的区域造成伤害,进而阻碍其生长。
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