The effect of experimental therapy on the gas and acid-base composition of blood in the dynamics of toxic pulmonary edema

Abstract

The present work is part of an extended research, which consisted in finding ways to prevent and treat toxic pulmonary edema. The purpose of this study was to study the gas transport function, acid-base state and gas composition of blood in the dynamics of experimental toxic pulmonary edema and the effect on them of experimental therapy with a combination of drugs that have shown their effectiveness in preliminary studies. Methods. The toxic edema of the lungs was modelled in mice by inhalation of toxic doses of phosgene LСt50. The parameters of the gas and acid-base composition of the blood were determined using a gas analyzer 3 and 24 hours after poisoning. Parts of the animals, 30 minutes after the poisoning, were injected intraperitoneally with a complex of drugs consisting of natrii dimercaptopropansulfonas (unitiol) 150 mg / kg, diclofenac sodium 35.0 mg / kg, and аprotinin (contrikal) 250 IU / kg. Results. It was found that 3 hours after poisoning in the blood of poisoned animals, changes in the gas composition of blood, parameters of the gas transport function of blood were detected, and 24 hours after poisoning a violation of the acid-base state. The use of a therapeutic combination of drugs consisting of sodium dimercaptopropanesulfonate (unithiol), sodium diclofenac and aprotinin (kontrikal) led to partial normalization of the altered parameters of the blood gas composition and parameters of the blood gas transport function, but was not accompanied by normalization of the acid-base state of the blood. The study of the effect of the formulation itself on the studied parameters of intact animals demonstrated a pronounced negative effect on the blood gas composition. Treatment of pulmonary edema with the comparison drug unithiol was not accompanied by normalization of the gas composition and acid-base state of the poisoned animals. Conclusion. Treatment of toxic pulmonary edema with a combination of medications led to an improvement in the gas composition and gas transport function of the blood. Keywords: pulmonary edema, phosgene, poisoning, blood gases, acid-base composition of blood, experimental treatment, unithiol, diclofenac sodium, aprotinin.