Genetic Diversity and Phylogenetic Relationships among Jatropha curcas L. Genotypes from Eastern India

Biotechnology Journal International Pub Date : 2023-11-20 DOI:10.9734/bji/2023/v27i6706

Sanjay Singh, Priyamvada Singh

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Abstract

Genetic diversity and relationships among 31 genotypes collected from wide geographical range in eastern India was studied employing Random Amplified Polymorphic DNA (RAPD) markers. 19 markers (identified out of 25) produced 139 band in total. Off these 139 bands, 131 bands were polymorphic exhibiting a high polymorphism of 94.24%. Similarity indices estimated on the basis of RAPD primers ranged widely from 0.44 to 0.83 which suggests that these accessions represent genetically diverse population possibly due to predominance of cross pollination and seed source variability. Genotyping data obtained for RAPD primer across collected accessions were used to generate the UPGMA - based phylogenetic tree which shows two major clusters. The cluster I consisted of 16 genotypes from two states Jharkhand and Odisha (Chotanagpur and Eastern plateau region) while 15 accessions from West Bengal and Bihar (Indo Gangetic plains region) were grouped together in the Cluster II. This clear alignment of accessions into two different clusters as per geographical regions was probably due to different growing conditions in two clusters.

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印度东部麻疯树基因型的遗传多样性和系统发育关系

利用随机扩增多态 DNA（RAPD）标记研究了从印度东部广泛地理范围收集的 31 个基因型之间的遗传多样性和关系。19 个标记（从 25 个标记中识别出）共产生 139 条带。在这 139 条带中，131 条带具有多态性，多态性高达 94.24%。根据 RAPD 引物估算的相似性指数在 0.44 到 0.83 之间，这表明这些品种代表了基因多样化的种群，这可能是由于杂交授粉和种子来源变异所造成的。通过 RAPD 引物获得的基因分型数据被用于生成基于 UPGMA 的系统发生树，该树显示出两个主要聚类。聚类 I 包括来自恰尔肯德邦和奥迪沙邦（乔塔纳格布尔和东部高原地区）的 16 个基因型，而聚类 II 则包括来自西孟加拉邦和比哈尔邦（印度恒河平原地区）的 15 个基因型。按地理区域将入选品种明确划分为两个不同的群组，可能是由于两个群组的生长条件不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biotechnology Journal International

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