Phytochemical investigation and in vitro cytotoxicity of Phlogacanthus thyrsiformis (Roxb. Ex Hardw.) Mabb. against Dalton’s Lymphoma Ascites (DLA) cells

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Mary Devi, Akalesh Kumar Verma, Namram Sushindrajit Singh, Jumi Das, Kasturi Dutta, Munmi Gogoi, Diksha Dutta
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引用次数: 0

Abstract

Phlogacanthus thyrsiformis, a valuable medicinal plant frequently employed by ethnic communities for various health concerns, has shown potential against cancer cell lines in lab tests. However, its effectiveness against Dalton’s Lymphoma (DL) cells remains unknown. In our current study, we aim to investigate the phytochemical constituents, antioxidant properties, and cytotoxic effects of P. thyrsiformis extract against the DL cell line. Additionally, we studied its apoptotic effects through fluorescent staining and Scanning Electron Micrograph (SEM) studies. The plant extract revealed the presence of various important phytochemicals such as carbohydrates, phenols, polyphenols, flavonoids, tannins, terpenoids, triterpenoids, alkaloids, and saponins in various degrees. The analysis revealed that the total phenol content and total flavonoid content were present in substantial quantities, suggesting a strong correlation with its potent antioxidant abilities and also showing close IC50 with the reference, ascorbic acid. Effective growth inhibition of DL cells was observed at different treatment concentrations without affecting normal cells. The occurrence of such cytotoxic effects could be corroborated to its apoptotic-inducing properties. Apoptotic cell death was observed in a dose-dependent manner and further morphological changes were evidenced through SEM studies. Our study thus substantiates that P. thyrsiformis is a potent plant with a reservoir of active phytochemicals and is a natural source of antitumor agents against DL cells.

Phlogacanthus thyrsiformis (Roxb. Ex Hardw.) Mabb.对道尔顿淋巴瘤腹水(DLA)细胞的植物化学研究和体外细胞毒性
Phlogacanthus thyrsiformis 是一种珍贵的药用植物,经常被少数民族用来治疗各种健康问题。然而,它对道尔顿淋巴瘤(DL)细胞的有效性仍然未知。在我们目前的研究中,我们旨在调查胸苷提取物的植物化学成分、抗氧化特性以及对 DL 细胞系的细胞毒性作用。此外,我们还通过荧光染色和扫描电子显微镜(SEM)研究了其凋亡效应。植物提取物中含有各种重要的植物化学物质,如不同程度的碳水化合物、酚类、多酚类、黄酮类、单宁、萜类、三萜类、生物碱和皂苷。分析表明,总酚含量和总黄酮含量都很高,这表明它具有很强的抗氧化能力,其 IC50 值也与抗坏血酸接近。在不同的处理浓度下,都能有效抑制 DL 细胞的生长,而不影响正常细胞。这种细胞毒性效应的出现可能与其诱导细胞凋亡的特性有关。凋亡细胞的死亡呈剂量依赖性,扫描电镜研究进一步证实了其形态学变化。因此,我们的研究证实,P. thyrsiformis 是一种有效的植物,含有大量活性植物化学物质,是抗 DL 细胞的天然抗肿瘤剂来源。
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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