Effect of Aqueous Leaves Extract of Datura alba ness (Thorn Apple) on Gonadotropic Hormones (FSH, LH) and Prolactin Following Administration of MSG and Clomiphene Citrate in Female Wistar Rats

Abstract

Phytomedicine has become popular among and majority of women depends on herbal medicine when seeking healthcare. Several women opted for herbal medicine when looking for fertility and most the women that hormonal imbalance depends on herbal medicine for correction. Hormones is substance that must be required and must be in the right values for reproduction to occur. The aim is this study is to ascertain the Effect of aqueous leave extract of D. alba ness (Thorn Apple) on gonadotropic hormones (FSH, LH) Treated with monosodium glutamate (MSG) and clomiphene citrate in female wistar rats. The animals were randomly selected into 7 with five animals per group. Each group received the following: group 1 10mls/kg of distil water, group 2 low dose of extract (1000mg/kg), group 3 high dose of extract(2000mg/kg), group 4 MSG 800mg/kg, group 5 clomid 50mg/kg, group 6 MSG + high dose of extract and group 7 clomid+ high dose of extract. Administration of extract was done for 21 days, and on the 22nd day, the animals were sacrificed and blood samples were collected for hormonal analysis. The results revealed significant decreased in the serum levels of follicle stimulating hormones (FSH) in all the treatment groups (extract 1000mg/kg, extract 2000mg/kg, MSG 800mg/kg, MSG 800mg/kg + extract 2000mg/kg, and clomid 50mg/kg + extract 2000mg/kg) when compared with control and significant increase in serum levels FSH in clomid 50mg/kg treated group. Luteinizing hormone (LH) serum levels significantly decreased in all the treatment groups when compared with the control group. LH levels in the clomid group significantly increased when compared with other treatment groups. The statistical analysis was carried out using Statistical Package for Social Science (SPSS) version 23.