The impact of Cortexin on cytokine levels in the treatment of moderate chronic generalized periodontitis in young and middle-aged people

I. B. Lkhasaranova, Y. Pinelis
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Abstract

Relevance. Chronic generalized periodontitis is one of the most common dental diseases in Russia and worldwide. Periodontitis causes changes in the immune system, cytokines, antioxidant protection, hemostasis and other systems, leading to periodontal microcirculatory and blood supply damage. Interleukins are regulatory molecules that play a part in specific immune reactions: inflammation, regeneration, and microcirculation. They are produced by immune system cells in response to an activating stimulus and regulate the strength and duration of immune and inflammatory reactions. Our study revealed an imbalance of pro- and anti-inflammatory cytokines in patients with moderate chronic generalized periodontitis of different ages.Material and Methods. The study involved 130 patients with moderate chronic generalized periodontitis aged 25 to 60. The control group consisted of 15 practically healthy individuals aged 18 to 24 years without acute and chronic periodontal diseases at the time of the study. The concentration of cytokines in blood serum and oral fluid (IL-1ß, IL-4, IL-8, IL-10) was evaluated in all patients before and after the standard comprehensive treatment and with the inclusion of Cortexin.Results. After the moderate chronic generalized periodontitis treatment, pro-inflammatory and anti-inflammatory cytokines in the blood decreased in the group of 25-44-year-olds. In standard treatment, IL-1ß level decreased by 30%, IL-4 – by two times, IL-8 – by 1.5 times, and IL-10 – by three times; but with Cortexin, IL-1ß became normal, IL-4 decreased by 3.5 times, IL-8 – by 2.5 times, IL-10 became normal. In the oral fluid of patients aged 25-44 years, IL-1ß decreased by 20% after the standard treatment, IL-4 decreased by six times, IL-8 decreased by two times, and the addition of Cortexin to the comprehensive therapy caused a 70% decrease, IL–4 reduced by ten times, IL-8 – by nine times, the concentration of IL-10 remained the same. IL-22 decreased by 1.5 and 2 times based on the treatment methods.The after-treatment assessment of changes in the interleukin content in blood serum in 45-60-year-old patients with chronic generalized periodontitis revealed the same levels of IL-4 after the standard treatment, a decrease of IL-1ß by 1.5 times, IL-8 - by 1.5 times; and the use of Cortexin in the treatment normalized the concentration of IL-4, led to a decrease in IL-1ß by two times, IL-8 - by three times. After the therapy, in the oral fluid of patients aged 45-60 years, IL-1ß decreased by 1.5 times with standard treatment, IL-4 and IL-8 decreased by two times, and IL-10 did not change, IL-22 - by 1.5 times; and using Cortexin, the concentration of IL-1ß decreased by 70%, IL-4 decreased by three times, IL-8 - by four times, IL-10 and IL-22 - by two times.Conclusion. Before treatment, the concentration of cytokines was higher in all groups of patients compared to the control values in blood serum and oral fluid. With standard therapy, the content of interleukins decreases in biological fluids, and the effect is more pronounced if standard treatment and Cortexin are combined. The correlation analysis detects that clinical manifestations of gingival inflammation are directly related to the concentration of pro- and anti-inflammatory interleukins in the blood and oral fluid.
在治疗中青年中度慢性全身性牙周炎的过程中,Cortexin 对细胞因子水平的影响
相关性。慢性全身性牙周炎是俄罗斯乃至全世界最常见的牙科疾病之一。牙周炎会引起免疫系统、细胞因子、抗氧化保护、止血和其他系统的变化,导致牙周微循环和供血受损。白细胞介素是一种调节分子,在特定的免疫反应中发挥作用:炎症、再生和微循环。它们由免疫系统细胞在激活刺激下产生,并调节免疫和炎症反应的强度和持续时间。我们的研究发现,不同年龄段的中度慢性泛发性牙周炎患者体内的促炎细胞因子和抗炎细胞因子失衡。研究涉及 130 名 25 至 60 岁的中度慢性泛发性牙周炎患者。对照组包括 15 名年龄在 18-24 岁之间、在研究期间没有急慢性牙周疾病的健康人。对所有患者在接受标准综合治疗前后和加入皮质素后血清和口腔液中细胞因子(IL-1ß、IL-4、IL-8、IL-10)的浓度进行了评估。中度慢性泛发性牙周炎治疗后,25-44 岁组患者血液中的促炎和抗炎细胞因子均有所下降。在标准治疗中,IL-1ß 水平降低了 30%,IL-4 降低了 2 倍,IL-8 降低了 1.5 倍,IL-10 降低了 3 倍;但在使用 Cortexin 后,IL-1ß 恢复正常,IL-4 降低了 3.5 倍,IL-8 降低了 2.5 倍,IL-10 恢复正常。在 25-44 岁患者的口腔液中,经过标准治疗后,IL-1ß 降低了 20%,IL-4 降低了 6 倍,IL-8 降低了 2 倍,而在综合疗法中添加柯特辛后,IL-1ß 降低了 70%,IL-4 降低了 10 倍,IL-8 降低了 9 倍,IL-10 的浓度保持不变。对 45-60 岁慢性全身性牙周炎患者血清中白细胞介素含量变化的治疗后评估显示,标准治疗后 IL-4 水平不变,IL-1ß 降低了 1.5 倍,IL-8 降低了 1.5 倍;在治疗中使用皮质素后,IL-4 的浓度恢复正常,IL-1ß 降低了 2 倍,IL-8 降低了 3 倍。治疗后,在 45-60 岁患者的口腔液中,IL-1ß 降低了 1.5 倍,IL-4 和 IL-8 降低了 2 倍,IL-10 没有变化,IL-22 降低了 1.5 倍;而使用 Cortexin 后,IL-1ß 的浓度降低了 70%,IL-4 降低了 3 倍,IL-8 降低了 4 倍,IL-10 和 IL-22 降低了 2 倍。治疗前,各组患者血清和口腔液中的细胞因子浓度均高于对照组。采用标准疗法后,生物液体中的白细胞介素含量会降低,如果标准疗法和可的松联合使用,效果会更明显。相关性分析表明,牙龈炎症的临床表现与血液和口腔液中促炎和抗炎白细胞介素的浓度直接相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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