Optimization of parameters for bio-ethanol production from sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice and finger millet malt using Taguchi method

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-29 DOI:10.4314/bcse.v38i1.5

Dolphene Okoth, Stephen Otieno, Francis Kiema, David Onyango, C. Kowenje

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Abstract

Bio-ethanol is a promising renewable energy but its production is expensive from high cost of feedstocks. In this study, sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice was investigated for bio-ethanol production. Most reports on bio-ethanol productions use commercial Saccharomyces cerevisiae as yeast. However, this study used finger millet (Eleusine coracana) malt with already high adaptation to local conditions and high economic viability as it is being utilized by the indigenous communities. Five sweet sorghum varieties of IESV-92001-DL (V1), NTJ (V2), 15233-IESV (V3), 92008-DJ (V4) and IESV-92028-DL (V5) were planted at 0°3'45.4644" North, 34°17'16.1052" South, in Kenya. °Brix content of juice was determined at 11th to 16th weeks after sowing. Highest °Brix for all varieties were at 15th week where V1 was highest at 22.07. V1 was then harvested for the juice. Factors affecting fermentation; temperature, time, pH and yeast to substrate ratio were optimized using Taguchi method and were obtained as 30 ℃, 48 hours, pH 5 and 5 g/L, respectively. Kinetics parameters of Vmax and Km were 0.35 g/L/h and 12.56 g/L, respectively. The optimized and kinetic parameters were within literature values and therefore finger millet malt has a great potential, as a substitute yeast source, in commercial bio-ethanol production. KEY WORDS: Bio-ethanol, Sweet sorghum juice, Taguchi method Bull. Chem. Soc. Ethiop. 2024, 38(1), 55-67. DOI: https://dx.doi.org/10.4314/bcse.v38i1.5

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用田口方法优化甜高粱（Sorghum bicolor (L.) Moench）茎汁和小米麦芽生产生物乙醇的参数

生物乙醇是一种前景广阔的可再生能源，但由于原料成本高昂，其生产成本也很高。本研究对甜高粱（Sorghum bicolor (L.) Moench）茎汁进行了生物乙醇生产研究。大多数关于生物乙醇生产的报告都使用商业酵母菌作为酵母。然而，这项研究使用的是指粟（Eleusine coracana）麦芽，这种麦芽对当地条件的适应性已经很强，而且经济可行性也很高，因为土著社区正在使用这种麦芽。在肯尼亚北纬 0°3'45.4644"、南纬 34°17'16.1052 "种植了五个甜高粱品种：IESV-92001-DL（V1）、NTJ（V2）、15233-IESV（V3）、92008-DJ（V4）和 IESV-92028-DL（V5）。果汁的 °Brix 含量在播种后第 11 至 16 周测定。所有品种在第 15 周时的白利糖度最高，其中 V1 的白利糖度最高，为 22.07。然后收获 V1 果汁。采用田口方法对影响发酵的因素：温度、时间、pH 值和酵母与底物的比例进行了优化，结果分别为 30 ℃、48 小时、pH 值 5 和 5 克/升。动力学参数 Vmax 和 Km 分别为 0.35 g/L/h 和 12.56 g/L。优化参数和动力学参数均在文献值范围内，因此，作为酵母源的替代品，小米麦芽在商业生物乙醇生产中具有很大的潜力。关键词：生物乙醇、甜高粱汁、田口法 Bull.Chem.Soc. Ethiop.2024, 38(1), 55-67.DOI: https://dx.doi.org/10.4314/bcse.v38i1.5

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.